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CDKN1A Antibody (C-term)Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Country
United States
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Ordering Information
Catalog # Size Availability Price  
AP13468b 400 µl (40 western blots) In Stock $ 265.00 Add to cart
AP13468b-ev 80 µl (8 western blots) In Stock $ 95.00 Add to cart
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CDKN1A Antibody (C-term) - Product info

ApplicationIF, WB, IHC
  • Applications Legend:
  • WB=Western Blotting
  • IP=Immunoprecipitation
  • IHC-P=Immunohistochemistry (Paraffin)
  • IF-IC=Immunofluorescence (Immunocytochemistry)
  • FC=Flow Cytometry
  • DB=Dot Blot
Primary AccessionP38936
Other AccessionNP_000380.1, NP_510867.1
ReactivityHuman, Mouse
IsotypeRabbit Ig
Calculated MW18119 Da

CDKN1A Antibody (C-term) - Additional info

Gene ID 1026
Other Names
CDKN1A; CAP20; CDKN1; CIP1; MDA6; PIC1; SDI1; WAF1; Cyclin-dependent kinase inhibitor 1; CDK-interacting protein 1; Melanoma differentiation-associated protein 6; p21
Target/Specificity
This CDKN1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 117-146 amino acids from the C-terminal region of human CDKN1A.
Dilution
IF~~1:10~50
WB~~1:1000
IHC~~1:10~50
Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions
CDKN1A Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.

CDKN1A Antibody (C-term) - Protein Information

Name CDKN1A
Synonyms CAP20, CDKN1, CIP1, MDA6, PIC1, SDI1, WA
Function
May be the important intermediate by which p53/TP53 mediates its role as an inhibitor of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin- dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D- CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex.
Cellular Location
Cytoplasm. Nucleus.
Tissue Location
Expressed in all adult tissues, with 5-fold lower levels observed in the brain

CDKN1A Antibody (C-term) - Related products

AP3875a: Phospho-mouse p21Cip1(S125) Antibody

AP3877a: Phospho-mouse p21Cip1(S78) Antibody

AM2134a: CDKN1A Antibody (C-term) (Ascites)

AM2134b: CDKN1A Antibody (C-term)

AP3187a: Phospho-p21Cip1(S130) Antibody

AP3188a: Phospho-P21CIP1(S146) Antibody

AP3189a: Phospho-P21CIP1(T145) Antibody

AP3190a: Phospho-P21CIP1(T57) Antibody

AP7527b: p21 (CDKN1A) Antibody (C-term)

AP9311a: P21Cip1 Antibody

BP13468b: CDKN1A Antibody (C-term) Blocking Peptides

BP13650b: CDKN1A Antibody (C-term) Blocking Peptides

BP3187a: Phospho-p21Cip1-pS130 Antibody Blocking Peptide

BP3188a: Phospho- P21CIP1-S146 Antibody Blocking Peptide

BP3189a: Phospho-P21CIP1-T145 Antibody Blocking Peptide

BP3190a: Phospho-P21CIP1-T57 Antibody Blocking Peptide

BP7527b: CDKN1A Antibody (C-term) Blocking Peptide

BP9311a: P21Cip1-S130 Antibody Blocking Peptide

AP20419b: Mouse p21Cip1 Antibody (C-term S148)

SP2035a: p21Cip1-T145 Phospho Peptide

SP2035b: Biotinylated p21Cip1-T145 Phospho Peptide

SP2035c: p21Cip1-T145 Non-phospho Control Peptide

SP2035d: Biotinylated p21Cip1-T145 Non-phospho Control Peptide

SP2036a: p21Cip1-S146 Phospho Peptide

SP2036b: Biotinylated p21Cip1-S146 Phospho Peptide

SP2036c: p21Cip1-S146 Non-phospho Control Peptide

SP2036d: Biotinylated p21Cip1-S146 Non-phospho Control Peptide

SP2037a: p21Cip1-T57 Phospho Peptide

SP2037b: Biotinylated p21Cip1-T57 Phospho Peptide

SP2037c: p21Cip1-T57 Non-phospho Control Peptide

SP2037d: Biotinylated p21Cip1-T57 Non-phospho Control Peptide

SP2175a: Cys - p21 (139 - 154)

SP2175b: Cys - p21 (139 - 154)

CDKN1A Antibody (C-term) - Application data

  • Fluorescent image of A549 cell stained with CDKN1A Antibody (C-term)(Cat#AP13468b).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with CDKN1A primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃).CDKN1A immunoreactivity is localized to Nucleus significantly.

  • CDKN1A Antibody (C-term) (Cat. #AP13468b) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the CDKN1A antibody detected the CDKN1A protein (arrow).

  • CDKN1A Antibody (C-term) (Cat. #AP13468b) western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).This demonstrates the CDKN1A antibody detected the CDKN1A protein (arrow).

  • CDKN1A Antibody (C-term) (Cat. #AP13468b)immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CDKN1A Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

CDKN1A Antibody (C-term) - Research Areas

AntibodiesStem CellsGene OntologyBiological ProcessOrgan DevelopmentCellular ProcessPositive Regulation Of Biological ProcessPositive Regulation Of Cellular ProcessRegulation Of Cell DeathRegulation Of Programmed Cell DeathResponse To Organic SubstanceRegulation Of ApoptosisRegulation Of PhosphorylationNegative Regulation Of Cellular ProcessNegative Regulation Of Biological ProcessResponse To Chemical StimulusRegulation Of Phosphate Metabolic ProcessRegulation Of Phosphorus Metabolic ProcessResponse To Endogenous StimulusSystem DevelopmentMulticellular Organismal DevelopmentAnatomical Structure DevelopmentRegulation Of Cell ProliferationResponse To Hormone StimulusNegative Regulation Of ApoptosisRegulation Of Kinase ActivityNegative Regulation Of Cell DeathNegative Regulation Of Programmed Cell DeathRegulation Of Protein Kinase ActivityDevelopmental ProcessRegulation Of Transferase ActivityRegulation Of Molecular FunctionRegulation Of Biological QualityPositive Regulation Of Cell DeathPositive Regulation Of Cell ProliferationPositive Regulation Of ApoptosisPositive Regulation Of Programmed Cell DeathRegulation Of Catalytic ActivityAnatomical Structure MorphogenesisResponse To Extracellular StimulusRegulation Of Cell CycleRegulation Of Immune System ProcessResponse To Steroid Hormone StimulusOrgan MorphogenesisResponse To StressNegative Regulation Of Metabolic ProcessNegative Regulation Of Cell ProliferationNegative Regulation Of Molecular FunctionCellular Response To StimulusNegative Regulation Of Cellular Metabolic ProcessResponse To External StimulusResponse To Oxygen LevelsCellular Response To Extracellular StimulusRegulation Of Metabolic ProcessNegative Regulation Of Catalytic ActivityInterphase Of Mitotic Cell CycleResponse To DrugMitotic Cell CycleInduction Of ApoptosisInterphaseInduction Of Programmed Cell DeathRegulation Of Cellular Metabolic ProcessRegulation Of Cell SizeNegative Regulation Of Cell CycleCellular Response To StressCell CycleResponse To Organic Cyclic SubstanceNegative Regulation Of PhosphorylationNegative Regulation Of Phosphate Metabolic ProcessNegative Regulation Of Phosphorus Metabolic ProcessNegative Regulation Of Kinase ActivityCell Cycle ProcessCell CommunicationResponse To Organic NitrogenNegative Regulation Of Transferase ActivityBiological RegulationRegulation Of GrowthRegulation Of Cellular Component SizeNegative Regulation Of Protein Kinase ActivityResponse To Abiotic StimulusResponse To Glucocorticoid StimulusResponse To Corticosteroid StimulusPositive Regulation Of Fibroblast ProliferationRegulation Of Leukocyte ProliferationRegulation Of Mononuclear Cell ProliferationRegulation Of Lymphocyte ProliferationG1/S Transition Of Mitotic Cell CycleCell Cycle PhaseRegulation Of Biological ProcessRegulation Of Fibroblast ProliferationResponse To Inorganic SubstanceMulticellular Organismal ProcessResponse To StimulusPositive Regulation Of Immune System ProcessCell Cycle ArrestRegulation Of Lymphocyte ActivationRegulation Of Cyclin-dependent Protein Kinase ActivityRegulation Of Cell ActivationRegulation Of Leukocyte ActivationResponse To RadiationRegulation Of Cellular ProcessRegulation Of Cell GrowthNegative Regulation Of Cell SizePositive Regulation Of Leukocyte ProliferationPositive Regulation Of Mononuclear Cell ProliferationRegenerationNegative Regulation Of GrowthPositive Regulation Of Lymphocyte ProliferationResponse To ToxinNegative Regulation Of Cell GrowthResponse To UVG2/M Transition Of Mitotic Cell CycleCellular CompartmentCytoplasmNucleusIntracellular PartIntracellularCytoplasmic PartMembrane-bounded OrganelleIntracellular Membrane-bounded OrganelleMembrane-enclosed LumenOrganelle LumenCytosolOrganelleIntracellular OrganelleIntracellular Organelle LumenOrganelle PartIntracellular Organelle PartNuclear LumenNuclear PartNucleoplasmMacromolecular ComplexProtein ComplexMolecular FunctionCatalytic ActivityKinase ActivityTransferase Activity, Transferring Phosphorus-containing GroupsTransferase ActivityProtein BindingProtein Complex BindingKinase Regulator ActivityPathwaysBiocartaCell Cycle G2/M CheckpointInfluence Of Ras And Rho Proteins On G1 To S TransitionKEGGCell CycleErbB Signaling PathwayProstate CancerGliomaMelanomaChronic Myeloid LeukemiaPathways In CancerBladder CancerP53 Signaling PathwayPantherP53 PathwayP53 Pathway Feedback Loops 2DiseaseBreast CancerLeukemiaProstate CancerOvarian CancerLung CancerBladder CancerOral CancerStomach CancerMelanomaTissueLungEyeEpitheliumAorta Endothelial Cell

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BACKGROUND

This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen (PCNA), a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Multiple alternatively spliced variants have been found for this gene.

REFERENCES

Hu, F., et al. Oncogene 29(40):5464-5474(2010) Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010) Jiang, P., et al. Acta Biochim. Biophys. Sin. (Shanghai) 42(9):671-676(2010) Ho-Pun-Cheung, A., et al. Pharmacogenomics J. (2010) In press : Do Nascimento Borges, B., et al. In Vivo 24(4):579-582(2010)