- CITATIONS: 3
|Application ||WB, IHC-P, IF, E|
|Other Accession||P20459, P68101, Q6ZWX6, P41374, Q5ZLX2, P68102, NP_004085.1, Q9I9E9|
|Predicted||Zebrafish, Bovine, Chicken, Drosophila, Rat, Yeast|
|Calculated MW||36112 Da|
|Antigen Region||60-89 aa|
|Other Names||Eukaryotic translation initiation factor 2 subunit 1, Eukaryotic translation initiation factor 2 subunit alpha, eIF-2-alpha, eIF-2A, eIF-2alpha, EIF2S1, EIF2A|
|Target/Specificity||This EIF2S1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 60-89 amino acids from the N-terminal region of human EIF2S1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||EIF2S1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.|
|Cellular Location||Cytoplasmic granule. Note=The cytoplasmic granules are stress granules which are a dense aggregation in the cytosol composed of proteins and RNAs that appear when the cell is under stress. Colocalizes with NANOS3 in the stress granules (By similarity).|
Provided below are standard protocols that you may find useful for product applications.
The translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).
Shiota, M., et al. Int. J. Oncol. 36(6):1521-1531(2010)
Kulkarni, A.P., et al. Indian J. Biochem. Biophys. 47(2):67-74(2010)
Wehner, K.A., et al. Mol. Cell. Biol. 30(8):2006-2016(2010)
Backes, S., et al. J. Gen. Virol. 91 (PT 2), 470-482 (2010) :
Sreejith, R.K., et al. Biochem. Biophys. Res. Commun. 390(2):273-279(2009)
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