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EIF2S1 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - EIF2S1 Antibody (N-term) AP13469a
    EIF2S1 Antibody (N-term) (Cat. #AP13469a) western blot analysis in mouse bladder tissue lysates (35ug/lane).This demonstrates the EIF2S1 antibody detected the EIF2S1 protein (arrow).
  • IF - EIF2S1 Antibody (N-term) AP13469a
    Confocal immunofluorescent analysis of EIF2S1 Antibody (N-term) (Cat#AP13469a) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
  • IHC-P - EIF2S1 Antibody (N-term) AP13469a
    EIF2S1 Antibody (N-term) (Cat. #AP13469a)immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of EIF2S1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P05198
Other Accession P20459, P68101, Q6ZWX6, P41374, Q5ZLX2, P68102, NP_004085.1, Q9I9E9
Reactivity Mouse
Predicted Rat, Chicken, Yeast, Bovine, Drosophila
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 36112 Da
Antigen Region 60-89 aa
Additional Information
Gene ID 1965
Other Names Eukaryotic translation initiation factor 2 subunit 1, Eukaryotic translation initiation factor 2 subunit alpha, eIF-2-alpha, eIF-2A, eIF-2alpha, EIF2S1, EIF2A
Target/Specificity This EIF2S1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 60-89 amino acids from the N-terminal region of human EIF2S1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsEIF2S1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name EIF2S1
Synonyms EIF2A
Function Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.
Cellular Location Cytoplasmic granule. Note=The cytoplasmic granules are stress granules which are a dense aggregation in the cytosol composed of proteins and RNAs that appear when the cell is under stress. Colocalizes with NANOS3 in the stress granules (By similarity).
Citations ( 0 )


The translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).


Shiota, M., et al. Int. J. Oncol. 36(6):1521-1531(2010)
Kulkarni, A.P., et al. Indian J. Biochem. Biophys. 47(2):67-74(2010)
Wehner, K.A., et al. Mol. Cell. Biol. 30(8):2006-2016(2010)
Backes, S., et al. J. Gen. Virol. 91 (PT 2), 470-482 (2010) :
Sreejith, R.K., et al. Biochem. Biophys. Res. Commun. 390(2):273-279(2009)

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$ 295.00
$ 99.00
Cat# AP13469a
(40 western blots)
Availability: In Stock
Bulk Size
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