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POU3F3 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - POU3F3 Antibody (Center) AP13495C
    POU3F3 Antibody (Center) (Cat. #AP13495c) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the POU3F3 antibody detected the POU3F3 protein (arrow).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P20264
Other Accession Q63262, P31361, P56222, P31360, P20265, P79745, NP_006227.1
Reactivity Human
Predicted Zebrafish, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 50327 Da
Antigen Region 294-323 aa
Additional Information
Gene ID 5455
Other Names POU domain, class 3, transcription factor 3, Brain-specific homeobox/POU domain protein 1, Brain-1, Brn-1, Octamer-binding protein 8, Oct-8, Octamer-binding transcription factor 8, OTF-8, POU3F3, BRN1, OTF8
Target/Specificity This POU3F3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 294-323 amino acids from the Central region of human POU3F3.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPOU3F3 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name POU3F3
Synonyms BRN1, OTF8
Function Transcription factor that acts synergistically with SOX11 and SOX4. Plays a role in neuronal development. Is implicated in an enhancer activity at the embryonic met- mesencephalic junction; the enhancer element contains the octamer motif (5'-ATTTGCAT-3') (By similarity).
Cellular Location Nucleus.
Tissue Location Brain.
Research Areas
Citations (0)

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POU3F3 is a member of the class III POU family of transcription factors (see POU3F1; MIM 602479) that are expressed in the central nervous system. The POU domain in these proteins is required for high affinity binding to octamer DNA sequences (summarized by Sumiyama et al., 1996 [PubMed 8703082]).[supplied by OMIM].


Wissmuller, S., et al. Nucleic Acids Res. 34(6):1735-1744(2006)
Kuhlbrodt, K., et al. J. Biol. Chem. 273(26):16050-16057(1998)
Sumiyama, K., et al. Mamm. Genome 9(2):180-181(1998)
Malik, K.F., et al. Brain Res. Mol. Brain Res. 45(1):99-107(1997)
Sumiyama, K., et al. J. Mol. Evol. 43(3):170-178(1996)

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$ 295.00
$ 99.00
Cat# AP13495C
(40 western blots)
Availability: In Stock
Bulk Size
Seasonal Special on Bulk Order
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