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CCDC99 Antibody (C-term)Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AP13504b | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
| AP13504b-ev20 | 20 ug 100 ul | In Stock | $ 95.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
- Reviews
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- Backgrounds
CCDC99 Antibody (C-term) - Product info | |
| Application | WB, IHC
|
| Primary Accession | Q96EA4 |
| Other Accession | Q4R7H3, NP_060255.3 |
| Reactivity | Human |
| Predicted | Monkey |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 70172 Da |
CCDC99 Antibody (C-term) - Additional info | |
| Gene ID 54908 | |
| Other Names CCDC99; Protein Spindly; Arsenite-related gene 1 protein; Coiled-coil domain-containing protein 99; Rhabdomyosarcoma antigen MU-RMS-40.4A | |
| Target/Specificity This CCDC99 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 458-488 amino acids from the C-terminal region of human CCDC99. | |
| Dilution WB~~1:100~500WB~~1:1000 IHC~~1:10~50 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions CCDC99 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. | |
CCDC99 Antibody (C-term) - Protein Information | |
| Name SPDL1 | |
| Synonyms CCDC99 | |
| Function Required for the localization of dynein and dynactin to the mitotic kintochore. Dynein is believed to control the initial lateral interaction between the kinetochore and spindle microtubules and to facilitate the subsequent formation of end-on kinetochore-microtubule attachments mediated by the NDC80 complex Also required for correct spindle orientation. Does not appear to be required for the removal of spindle assembly checkpoint (SAC) proteins from the kinetochore upon bipolar spindle attachment | |
| Cellular Location Cytoplasm, cytoskeleton, centrosome. Chromosome, centromere, kinetochore. Nucleus. Cytoplasm, cytoskeleton, spindle pole. Note=Localizes to the nucleus in interphase and to the kinetochore in early prometaphase Relocalizes to the mitotic spindle pole before metaphase and is subsequently lost from the spindle poles after chromosome congression is completed. Removal of this protein from the kinetochore requires the dynein/dynactin complex | |
CCDC99 Antibody (C-term) - Related products
CCDC99 Antibody (C-term) - Application data
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CCDC99 Antibody (C-term) (Cat. #AP13504b) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the CCDC99 antibody detected the CCDC99 protein (arrow).
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CCDC99 Antibody (C-term) (Cat. #AP13504b)immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CCDC99 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
CCDC99 Antibody (C-term) - Research Areas
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BACKGROUND
CCDC99 is required for the localization of dynein and dynactin to the mitotic kintochore. Dynein is believed to control the initial lateral interaction between the kinetochore and spindle microtubules and to facilitate the subsequent formation of end-on kinetochore-microtubule attachments mediated by the NDC80 complex. Also required for correct spindle orientation. Does not appear to be required for the removal of spindle assembly checkpoint (SAC) proteins from the kinetochore upon bipolar spindle attachment.
REFERENCES
Barisic, M., et al. Mol. Biol. Cell 21(12):1968-1981(2010) Gassmann, R., et al. Genes Dev. 24(9):957-971(2010) Chan, Y.W., et al. J. Cell Biol. 185(5):859-874(2009)
