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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   CCDC99 Antibody (C-term)   

CCDC99 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - CCDC99 Antibody (C-term) AP13504b
    CCDC99 Antibody (C-term) (Cat. #AP13504b) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the CCDC99 antibody detected the CCDC99 protein (arrow).
  • IHC-P - CCDC99 Antibody (C-term) AP13504b
    CCDC99 Antibody (C-term) (Cat. #AP13504b)immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CCDC99 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession Q96EA4
Other Accession Q4R7H3, NP_060255.3
Reactivity Human
Predicted Monkey
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 70172 Da
Antigen Region 459-488 aa
Additional Information
Gene ID 54908
Other Names Protein Spindly {ECO:0000255|HAMAP-Rule:MF_03041}, hSpindly, Arsenite-related gene 1 protein, Coiled-coil domain-containing protein 99 {ECO:0000255|HAMAP-Rule:MF_03041}, Rhabdomyosarcoma antigen MU-RMS-404A, Spindle apparatus coiled-coil domain-containing protein 1 {ECO:0000255|HAMAP-Rule:MF_03041}, SPDL1 {ECO:0000255|HAMAP-Rule:MF_03041}
Target/Specificity This CCDC99 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 459-488 amino acids from the C-terminal region of human CCDC99.
Dilution WB~~1:1000
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCCDC99 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SPDL1 {ECO:0000255|HAMAP-Rule:MF_03041}
Function Required for the localization of dynein and dynactin to the mitotic kintochore. Dynein is believed to control the initial lateral interaction between the kinetochore and spindle microtubules and to facilitate the subsequent formation of end-on kinetochore-microtubule attachments mediated by the NDC80 complex. Also required for correct spindle orientation. Does not appear to be required for the removal of spindle assembly checkpoint (SAC) proteins from the kinetochore upon bipolar spindle attachment (PubMed:17576797, PubMed:19468067). Acts as an adapter protein linking the dynein motor complex to various cargos and converts dynein from a non-processive to a highly processive motor in the presence of dynactin. Facilitates the interaction between dynein and dynactin and activates dynein processivity (the ability to move along a microtubule for a long distance without falling off the track) (PubMed:25035494).
Cellular Location Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Chromosome, centromere, kinetochore. Nucleus. Cytoplasm, cytoskeleton, spindle pole Note=Localizes to the nucleus in interphase and to the kinetochore in early prometaphase. Relocalizes to the mitotic spindle pole before metaphase and is subsequently lost from the spindle poles after chromosome congression is completed. Removal of this protein from the kinetochore requires the dynein/dynactin complex
Research Areas
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Background

CCDC99 is required for the localization of dynein and dynactin to the mitotic kintochore. Dynein is believed to control the initial lateral interaction between the kinetochore and spindle microtubules and to facilitate the subsequent formation of end-on kinetochore-microtubule attachments mediated by the NDC80 complex. Also required for correct spindle orientation. Does not appear to be required for the removal of spindle assembly checkpoint (SAC) proteins from the kinetochore upon bipolar spindle attachment.

References

Barisic, M., et al. Mol. Biol. Cell 21(12):1968-1981(2010)
Gassmann, R., et al. Genes Dev. 24(9):957-971(2010)
Chan, Y.W., et al. J. Cell Biol. 185(5):859-874(2009)

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Cat# AP13504b
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