|Application ||WB, IHC-P, E|
|Calculated MW||28192 Da|
|Antigen Region||7-36 aa|
|Other Names||C->U-editing enzyme APOBEC-1, 354-, Apolipoprotein B mRNA-editing enzyme 1, HEPR, APOBEC1|
|Target/Specificity||This Apobec1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 7-36 amino acids from the N-terminal region of human Apobec1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Apobec1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Catalytic component of the apolipoprotein B mRNA editing enzyme complex which is responsible for the postranscriptional editing of a CAA codon for Gln to a UAA codon for stop in the APOB mRNA. Also involved in CGA (Arg) to UGA (Stop) editing in the NF1 mRNA. May also play a role in the epigenetic regulation of gene expression by participating in DNA demethylation.|
|Tissue Location||Expressed exclusively in the small intestine.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
APOBEC1 is involved in the production of apolipoprotein B (apoB)-48 from apoB-100. The gene spans 18 kb and contains five exons, all of which are translated. Alternative splicing produces a variant transcript that lacks exon 2 and encodes a novel 36-amino acid peptide. The exon 2-skipped transcript accounts for approximately 50% of APOBEC1 mRNA in the adult small intestine and up to 90% of APOBEC1 mRNA in the developing gut. Exon 2-skipping may thus be a quantitatively important mechanism for regulating the expression of this gene in the gastrointestinal tract.
Blanc, V., et al., J. Biol. Chem. 278(42):41198-41204 (2003).
Chester, A., et al., EMBO J. 22(15):3971-3982 (2003).
Wedekind, J.E., et al., Trends Genet. 19(4):207-216 (2003).
Mukhopadhyay, D., et al., Am. J. Hum. Genet. 70(1):38-50 (2002).
Dance, G.S., et al., J. Biol. Chem. 277(15):12703-12709 (2002).
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