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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   CD33 Antibody (Center)   

CD33 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - CD33 Antibody (Center) AP13541C
    CD33 Antibody (Center) (Cat. #AP13541c) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the CD33 antibody detected the CD33 protein (arrow).
  • WB - CD33 Antibody (Center) AP13541C
    Western blot analysis of CD33 (arrow) using rabbit polyclonal CD33 Antibody (Center) (Cat. #AP13541c). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CD33 gene.
  • SPECIFICATION
  • CITATIONS
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P20138
Other Accession NP_001763.3
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 39825 Da
Antigen Region 88-117 aa
Additional Information
Gene ID 945
Other Names Myeloid cell surface antigen CD33, Sialic acid-binding Ig-like lectin 3, Siglec-3, gp67, CD33, CD33, SIGLEC3
Target/Specificity This CD33 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 88-117 amino acids from the Central region of human CD33.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCD33 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CD33
Synonyms SIGLEC3
Function Putative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. Induces apoptosis in acute myeloid leukemia (in vitro).
Cellular Location Cell membrane; Single-pass type I membrane protein
Tissue Location Monocytic/myeloid lineage cells.
Research Areas
Citations (0)

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Background

Putative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. Induces apoptosis in acute myeloid leukemia (in vitro).

References

Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Davila, S., et al. Genes Immun. 11(3):232-238(2010)
Akahane, K., et al. Leukemia 24(4):865-869(2010)
Shamsasenjan, K., et al. Int. J. Hematol. 89(3):310-318(2009)
Bertram, L., et al. Am. J. Hum. Genet. 83(5):623-632(2008)

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Cat# AP13541C
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(40 western blots)
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