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ESR1/ER Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - ESR1/ER Antibody (Center) AP13547c
    All lanes : Anti-ESR1/ER Antibody (Center) at 1:1000 dilution Lane 1: T47D whole cell lysate Lane 2: MCF-7 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 66 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - ESR1/ER Antibody (Center) AP13547c
    ESR1/ER Antibody (Center) (Cat. #AP13547c) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the ESR1/ER antibody detected the ESR1/ER protein (arrow).
  • IHC-P - ESR1/ER Antibody (Center) AP13547c
    ESR1/ER Antibody (Center) (Cat. #AP13547c)immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ESR1/ER Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
  • IHC-P - ESR1/ER Antibody (Center) AP13547c
    ESR1/ER Antibody (Center) (Cat. #AP13547c)immunohistochemistry analysis in formalin fixed and paraffin embedded human uterus tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ESR1/ER Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
  • IF - ESR1/ER Antibody (Center) AP13547c
    Confocal immunofluorescent analysis of ESR1/ER Antibody (Center)(Cat#AP13547c) with ZR-75-1 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P03372
Other Accession P06211, Q29040, P19785, P49884, NP_001116213.1, NP_000116.2, NP_001116214.1, NP_001116212.1, Q9TV98
Reactivity Human
Predicted Mouse, Rat, Pig, Bovine
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 66216 Da
Antigen Region 246-273 aa
Additional Information
Gene ID 2099
Other Names Estrogen receptor, ER, ER-alpha, Estradiol receptor, Nuclear receptor subfamily 3 group A member 1, ESR1, ESR, NR3A1
Target/Specificity This ESR1/ER antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 246-273 amino acids from the Central region of human ESR1/ER.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsESR1/ER Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ESR1
Synonyms ESR, NR3A1
Function Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Ligand-dependent nuclear transactivation involves either direct homodimer binding to a palindromic estrogen response element (ERE) sequence or association with other DNA- binding transcription factors, such as AP-1/c-Jun, c-Fos, ATF-2, Sp1 and Sp3, to mediate ERE-independent signaling. Ligand binding induces a conformational change allowing subsequent or combinatorial association with multiprotein coactivator complexes through LXXLL motifs of their respective components. Mutual transrepression occurs between the estrogen receptor (ER) and NF- kappa-B in a cell-type specific manner. Decreases NF-kappa-B DNA- binding activity and inhibits NF-kappa-B-mediated transcription from the IL6 promoter and displace RELA/p65 and associated coregulators from the promoter. Recruited to the NF-kappa-B response element of the CCL2 and IL8 promoters and can displace CREBBP. Present with NF-kappa-B components RELA/p65 and NFKB1/p50 on ERE sequences. Can also act synergistically with NF-kappa-B to activate transcription involving respective recruitment adjacent response elements; the function involves CREBBP. Can activate the transcriptional activity of TFF1. Also mediates membrane-initiated estrogen signaling involving various kinase cascades. Isoform 3 is involved in activation of NOS3 and endothelial nitric oxide production. Isoforms lacking one or several functional domains are thought to modulate transcriptional activity by competitive ligand or DNA binding and/or heterodimerization with the full length receptor. Essential for MTA1-mediated transcriptional regulation of BRCA1 and BCAS3. Isoform 3 can bind to ERE and inhibit isoform 1.
Cellular Location Isoform 1: Nucleus {ECO:0000255|PROSITE- ProRule:PRU00407, ECO:0000269|PubMed:12682286}. Cytoplasm. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Note=A minor fraction is associated with the inner membrane Nucleus. Golgi apparatus. Cell membrane. Note=Colocalizes with ZDHHC7 and ZDHHC21 in the Golgi apparatus where most probably palmitoylation occurs. Associated with the plasma membrane when palmitoylated
Tissue Location Widely expressed. Isoform 3 is not expressed in the pituitary gland.
Research Areas
Citations ( 0 )


This gene encodes an estrogen receptor, a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative splicing results in several transcript variants, which differ in their 5' UTRs and use different promoters.


Geradts, J., et al. Cancer Invest. 28(9):969-977(2010)
Hayes, D.F., et al. Clin. Pharmacol. Ther. 88(5):626-629(2010)
Lupien, M., et al. Genes Dev. 24(19):2219-2227(2010)
Corbo, R.M., et al. J. Gerontol. A Biol. Sci. Med. Sci. (2010) In press :
Kim, S., et al. Fertil. Steril. (2010) In press :

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$ 295.00
$ 99.00
Cat# AP13547c
(40 western blots)
Availability: In Stock
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