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RNASE3 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - RNASE3 Antibody (C-term) AP13557b
    Western blot analysis of lysate from human spleen tissue lysate, using RNASE3 Antibody (C-term)(Cat. #AP13557b). AP13557b was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 35ug.
  • WB - RNASE3 Antibody (C-term) AP13557b
    RNASE3 Antibody (C-term) (Cat. #AP13557b) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the RNASE3 antibody detected the RNASE3 protein (arrow).
  • IF - RNASE3 Antibody (C-term) AP13557b
    Confocal immunofluorescent analysis of RNASE3 Antibody (C-term) (Cat#AP13557b) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF, E
Primary Accession P12724
Other Accession NP_002926.2
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 18385 Da
Antigen Region 124-151 aa
Additional Information
Gene ID 6037
Other Names Eosinophil cationic protein, ECP, 3127-, Ribonuclease 3, RNase 3, RNASE3, ECP, RNS3
Target/Specificity This RNASE3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 124-151 amino acids from the C-terminal region of human RNASE3.
Dilution WB~~1:1000
IF~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRNASE3 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RNASE3
Synonyms ECP, RNS3
Function Cytotoxin and helminthotoxin with low-efficiency ribonuclease activity. Possesses a wide variety of biological activities. Exhibits antibacterial activity, including cytoplasmic membrane depolarization of preferentially Gram-negative, but also Gram-positive strains. Promotes E.coli outer membrane detachment, alteration of the overall cell shape and partial loss of cell content.
Cellular Location Secreted. Note=Located in the matrix of eosinophil large specific granule, which are released following activation by an immune stimulus
Citations (0)

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Background

Cytotoxin and helminthotoxin with low-efficiency ribonuclease activity. Possesses a wide variety of biological activities. Exhibits antibacterial activity.

References

Peona, V., et al. J Asthma 47(2):131-134(2010)
Kang, I., et al. Eur Arch Otorhinolaryngol 267(3):391-395(2010)
Jonsson, U.B., et al. BMC Pulm Med 10, 36 (2010) :
Kato, M., et al. Int. Arch. Allergy Immunol. 152 SUPPL 1, 62-66 (2010) :
Chang, K.C., et al. BMC Cell Biol. 11, 6 (2010) :

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$ 295.00
$ 99.00
Cat# AP13557b
Size:
Quantity:
(40 western blots)
Availability: In Stock
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