|Application ||WB, IHC-P, E|
|Calculated MW||43671 Da|
|Antigen Region||168-197 aa|
|Other Names||Potassium channel subfamily K member 18, TWIK-related individual potassium channel, TWIK-related spinal cord potassium channel, KCNK18, TRESK, TRIK|
|Target/Specificity||This KCNK18 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 168-197 amino acids from the Central region of human KCNK18.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||KCNK18 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Outward rectifying potassium channel. Produces rapidly activating outward rectifier K(+) currents. May function as background potassium channel that sets the resting membrane potential. Channel activity is directly activated by calcium signal. Activated by the G(q)-protein coupled receptor pathway. The calcium signal robustly activates the channel via calcineurin, whereas the anchoring of 14-3-3/YWHAH interferes with the return of the current to the resting state after activation. Inhibited also by arachidonic acid and other naturally occurring unsaturated free fatty acids. Channel activity is also enhanced by volatile anesthetics, such as isoflurane. Appears to be the primary target of hydroxy-alpha-sanshool, an ingredient of Schezuan pepper. May be involved in the somatosensory function with special respect to pain sensation (By similarity).|
|Cellular Location||Cell membrane; Multi-pass membrane protein|
|Tissue Location||Expressed specifically in dorsal root ganglion and trigeminal ganglion neurons. Detected at low levels in spinal cord.|
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Provided below are standard protocols that you may find useful for product applications.
Two-pore domain potassium channels, such as KCNK18, give rise to background, or leak, potassium conductance, and they regulate diverse cellular functions by adjusting both the resting membrane potential and excitability. KCNK18 is unique among this family of potassium channels in that its activity is regulated by intracellular calcium (summary by Czirjak and Enyedi, 2006 [PubMed 16569637]).
Lafreniere, R.G., et al. Nat. Med. 16(10):1157-1160(2010)
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Egenberger, B., et al. Biochem. Biophys. Res. Commun. 391(2):1262-1267(2010)
Pottosin, I.I., et al. Pflugers Arch. 456(6):1037-1048(2008)
Czirjak, G., et al. J. Biol. Chem. 283(23):15672-15680(2008)
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