|Application ||WB, IHC-P, E|
|Other Accession||Q9QUS6, NP_036348.2, NP_438112.2|
|Calculated MW||83210 Da|
|Antigen Region||690-717 aa|
|Other Names||Probable E3 ubiquitin-protein ligase MID2, 632-, Midin-2, Midline defect 2, Midline-2, RING finger protein 60, Tripartite motif-containing protein 1, MID2, FXY2, RNF60, TRIM1|
|Target/Specificity||This MID2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 690-717 amino acids from the C-terminal region of human MID2.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||MID2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||FXY2, RNF60, TRIM1|
|Function||May play a role in microtubule stabilization.|
|Cellular Location||Cytoplasm. Cytoplasm, cytoskeleton. Note=Microtubule-associated|
|Tissue Location||Low level in fetal kidney and lung, and in adult prostate, ovary and small intestine|
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Provided below are standard protocols that you may find useful for product applications.
The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to microtubular structures in the cytoplasm. Alternate splicing of this gene results in two transcript variants encoding different isoforms.
Lim, J., et al. Cell 125(4):801-814(2006)
Jehee, F.S., et al. Am. J. Med. Genet. A 139(3):221-226(2005)
Yap, M.W., et al. Proc. Natl. Acad. Sci. U.S.A. 101(29):10786-10791(2004)
Short, K.M., et al. BMC Cell Biol. 3, 1 (2002) :
Reymond, A., et al. EMBO J. 20(9):2140-2151(2001)
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