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MID2 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - MID2 Antibody (C-term) AP13639b
    MID2 Antibody (C-term) (Cat. #AP13639b) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the MID2 antibody detected the MID2 protein (arrow).
  • IHC-P - MID2 Antibody (C-term) AP13639b
    MID2 Antibody (C-term) (Cat. #AP13639b)immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MID2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9UJV3
Other Accession Q9QUS6, NP_036348.2, NP_438112.2
Reactivity Human
Predicted Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 83210 Da
Antigen Region 690-717 aa
Additional Information
Gene ID 11043
Other Names Probable E3 ubiquitin-protein ligase MID2, 632-, Midin-2, Midline defect 2, Midline-2, RING finger protein 60, Tripartite motif-containing protein 1, MID2, FXY2, RNF60, TRIM1
Target/Specificity This MID2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 690-717 amino acids from the C-terminal region of human MID2.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMID2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MID2
Synonyms FXY2, RNF60, TRIM1
Function May play a role in microtubule stabilization.
Cellular Location Cytoplasm. Cytoplasm, cytoskeleton. Note=Microtubule-associated
Tissue Location Low level in fetal kidney and lung, and in adult prostate, ovary and small intestine
Research Areas
Citations (0)

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The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to microtubular structures in the cytoplasm. Alternate splicing of this gene results in two transcript variants encoding different isoforms.


Lim, J., et al. Cell 125(4):801-814(2006)
Jehee, F.S., et al. Am. J. Med. Genet. A 139(3):221-226(2005)
Yap, M.W., et al. Proc. Natl. Acad. Sci. U.S.A. 101(29):10786-10791(2004)
Short, K.M., et al. BMC Cell Biol. 3, 1 (2002) :
Reymond, A., et al. EMBO J. 20(9):2140-2151(2001)

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Cat# AP13639b
(40 western blots)
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