TRIM15 Antibody (Center)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, E |
---|---|
Primary Accession | Q9C019 |
Other Accession | NP_150232.2 |
Reactivity | Human |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 52113 Da |
Antigen Region | 247-276 aa |
Gene ID | 89870 |
---|---|
Other Names | Tripartite motif-containing protein 15, RING finger protein 93, Zinc finger protein 178, Zinc finger protein B7, TRIM15, RNF93, ZNF178, ZNFB7 |
Target/Specificity | This TRIM15 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 247-276 amino acids from the Central region of human TRIM15. |
Dilution | WB~~1:1000 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | TRIM15 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | TRIM15 |
---|---|
Synonyms | RNF93, ZNF178, ZNFB7 |
Function | E3 ubiquitin ligase that plays a role in several processes including innate antiviral immnity, cell migration and chemotaxis (PubMed:34142270, PubMed:23077300). Acts as a 'Lys-63'-specific ubiquitin ligase for MAPK1/ERK2 and MAPK3/ERK1, promoting their activation by facilitating their interaction with MAP2K1 and MAP2K2 (PubMed:34497368). Plays also a role in cell migration and chemotaxis by acting as a stable focal adhesion component upon recruitment by multi-adapter protein paxillin/PXN (PubMed:25015296). Functions in the RIGI-mediated interferon induction pathway upstream or at the level of MAVS (PubMed:23077300). Inhibits NF-kappa-B activation by turnover of 'Lys-63'-linked ubiquitination of MAP3K7/TAK1. Mechanistically, prevents TRIM8 cytoplasmic translocation and thus inhibits TRIM8- mediated 'Lys-63'-linked polyubiquitination of MAP3K7/TAK1 in the cytoplasm (PubMed:34871740). Plays also an important regulatory effect on the activation of hepatic stellate cells (HSCs). |
Cellular Location | Cytoplasm. Nucleus Cell junction, focal adhesion Note=Localizes to focal adhesions during the early stage of adhesion biogenesis. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
info@abcepta.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
Background
The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to the cytoplasm. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq].
References
McElroy, J.P., et al. Hum. Mol. Genet. 19(15):3080-3088(2010)
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Barcellos, L.F., et al. PLoS Genet. 5 (10), E1000696 (2009) :
Uchil, P.D., et al. PLoS Pathog. 4 (2), E16 (2008) :
Lamesch, P., et al. Genomics 89(3):307-315(2007)
If you have used an Abcepta product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at tech@abcepta.com.