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TRIM23 Antibody (N-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| IHC-P, WB, E |
|---|---|
| Primary Accession | P36406 |
| Other Accession | P36407, Q8BGX0, NP_150230.1, NP_001647.1, NP_150231.1 |
| Reactivity | Human |
| Predicted | Mouse, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 64067 Da |
| Antigen Region | 147-175 aa |
| Gene ID | 373 |
|---|---|
| Other Names | E3 ubiquitin-protein ligase TRIM23, 632-, ADP-ribosylation factor domain-containing protein 1, GTP-binding protein ARD-1, RING finger protein 46, Tripartite motif-containing protein 23, TRIM23, ARD1, ARFD1, RNF46 |
| Target/Specificity | This TRIM23 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 147-175 amino acids from the N-terminal region of human TRIM23. |
| Dilution | WB~~1:1000 IHC-P~~1:10~50 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | TRIM23 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | TRIM23 |
|---|---|
| Synonyms | ARD1, ARFD1, RNF46 |
| Function | Acts as an E3 ubiquitin-protein ligase. Plays an essential role in autophagy activation during viral infection. Mechanistically, activates TANK-binding kinase 1/TBK1 by facilitating its dimerization and ability to phosphorylate the selective autophagy receptor SQSTM1. In order to achieve this function, TRIM23 mediates 'Lys-27'-linked auto-ubiquitination of its ADP-ribosylation factor (ARF) domain to induce its GTPase activity and its recruitment to autophagosomes (PubMed:28871090). |
| Cellular Location | Cytoplasm. Endomembrane system. Golgi apparatus membrane. Lysosome membrane. Note=Membrane-associated with the Golgi complex and lysosomal structures |
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Provided below are standard protocols that you may find useful for product applications.
Background
The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein is also a member of the ADP ribosylation factor family of guanine nucleotide-binding family of proteins. Its carboxy terminus contains an ADP-ribosylation factor domain and a guanine nucleotide binding site, while the amino terminus contains a GTPase activating protein domain which acts on the guanine nucleotide binding site. The protein localizes to lysosomes and the Golgi apparatus. It plays a role in the formation of intracellular transport vesicles, their movement from one compartment to another, and phopholipase D activation. Three alternatively spliced transcript variants for this gene have been described.
References
Arimoto, K., et al. Proc. Natl. Acad. Sci. U.S.A. 107(36):15856-15861(2010)
Poole, E., et al. J. Virol. 83(8):3581-3590(2009)
Venkatesan, K., et al. Nat. Methods 6(1):83-90(2009)
Vichi, A., et al. Proc. Natl. Acad. Sci. U.S.A. 102(6):1945-1950(2005)
Reymond, A., et al. EMBO J. 20(9):2140-2151(2001)
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