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PPP1R3F Antibody (C-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - PPP1R3F Antibody (C-term) AP13676b
    Anti-PPP1R3F Antibody (C-term) at 1:1000 dilution + human heart lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 83 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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  • WB - PPP1R3F Antibody (C-term) AP13676b
    PPP1R3F Antibody (C-term) (Cat. #AP13676b) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the PPP1R3F antibody detected the PPP1R3F protein (arrow).
    detail
  • IHC-P - PPP1R3F Antibody (C-term) AP13676b
    PPP1R3F Antibody (C-term) (Cat. #AP13676b)immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PPP1R3F Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-P, WB, E
Primary Accession Q6ZSY5
Other Accession Q9JIG4, NP_149992.3
Reactivity Human
Predicted Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 82798 Da
Antigen Region 549-578 aa
Additional Information
Gene ID 89801
Other Names Protein phosphatase 1 regulatory subunit 3F, R3F, PPP1R3F
Target/Specificity This PPP1R3F antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 549-578 amino acids from the C-terminal region of human PPP1R3F.
Dilution WB~~1:1000
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPPP1R3F Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PPP1R3F
Function Glycogen-targeting subunit for protein phosphatase 1 (PP1).
Cellular Location Membrane; Single-pass membrane protein
Tissue Location Expressed in brain, skeletal muscle and heart.
Research Areas
Citations (0)
citation

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Background

This gene encodes a protein that has been identified as one of several type-1 protein phosphatase (PP1) regulatory subunits. One or two of these subunits, together with the well-conserved catalytic subunit, can form the PP1 holoenzyme, where the regulatory subunit functions to regulate substrate specificity and/or targeting to a particular cellular compartment. Alternative splicing results in multiple transcript variants.

References

Suttner, K., et al. J. Allergy Clin. Immunol. 125(6):1395-1399(2010)
Piton, A., et al. Mol. Psychiatry (2010) In press :
Ceulemans, H., et al. Bioessays 24(4):371-381(2002)
Schindelhauer, D., et al. Genome Res. 6(11):1056-1069(1996)

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$ 365.00
$ 140.00
Cat# AP13676b
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