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P4HA3 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - P4HA3 Antibody (Center) AP13685c
    P4HA3 Antibody (Center) (Cat. #AP13685c) western blot analysis in human placenta tissue lysates (35ug/lane).This demonstrates the P4HA3 antibody detected the P4HA3 protein (arrow).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q7Z4N8
Other Accession NP_878907.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 61126 Da
Antigen Region 274-303 aa
Additional Information
Gene ID 283208
Other Names Prolyl 4-hydroxylase subunit alpha-3, 4-PH alpha-3, Procollagen-proline, 2-oxoglutarate-4-dioxygenase subunit alpha-3, P4HA3
Target/Specificity This P4HA3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 274-303 amino acids from the Central region of human P4HA3.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsP4HA3 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name P4HA3
Function Catalyzes the post-translational formation of 4- hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins.
Cellular Location Endoplasmic reticulum lumen.
Tissue Location Highly expressed in placenta, liver and fetal skin. Weakly expressed in fetal epiphyseal cartilage, fetal liver, fibroblast, lung and skeletal muscle. Expressed also in fibrous cap of carotid atherosclerotic lesions
Research Areas
Citations (0)

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This gene encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein is one of several different types of alpha subunits and provides the major part of the catalytic site of the active enzyme. In collagen and related proteins, prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline that is essential to the proper three-dimensional folding of newly synthesized procollagen chains. Alternatively spliced transcript variants have been observed, but their full-length nature has not been determined.


Rose, J. Phd, et al. Mol. Med. (2010) In press :
Koivunen, P., et al. J. Biol. Chem. 281(39):28712-28720(2006)
Kukkola, L., et al. J. Biol. Chem. 278(48):47685-47693(2003)
Van Den Diepstraten, C., et al. Circulation 108(5):508-511(2003)

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$ 295.00
$ 99.00
Cat# AP13685c
(40 western blots)
Availability: In Stock
Bulk Size
Seasonal Special on Bulk Order
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