|Application ||WB, E|
|Calculated MW||61126 Da|
|Antigen Region||274-303 aa|
|Other Names||Prolyl 4-hydroxylase subunit alpha-3, 4-PH alpha-3, Procollagen-proline, 2-oxoglutarate-4-dioxygenase subunit alpha-3, P4HA3|
|Target/Specificity||This P4HA3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 274-303 amino acids from the Central region of human P4HA3.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||P4HA3 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Catalyzes the post-translational formation of 4- hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins.|
|Cellular Location||Endoplasmic reticulum lumen.|
|Tissue Location||Highly expressed in placenta, liver and fetal skin. Weakly expressed in fetal epiphyseal cartilage, fetal liver, fibroblast, lung and skeletal muscle. Expressed also in fibrous cap of carotid atherosclerotic lesions|
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Provided below are standard protocols that you may find useful for product applications.
This gene encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein is one of several different types of alpha subunits and provides the major part of the catalytic site of the active enzyme. In collagen and related proteins, prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline that is essential to the proper three-dimensional folding of newly synthesized procollagen chains. Alternatively spliced transcript variants have been observed, but their full-length nature has not been determined.
Rose, J. Phd, et al. Mol. Med. (2010) In press :
Koivunen, P., et al. J. Biol. Chem. 281(39):28712-28720(2006)
Kukkola, L., et al. J. Biol. Chem. 278(48):47685-47693(2003)
Van Den Diepstraten, C., et al. Circulation 108(5):508-511(2003)
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