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>   home   >   Products   >   Primary Antibodies   >   HIST1H1E Antibody (N-term)   

HIST1H1E Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - HIST1H1E Antibody (N-term) AP13710a
    HIST1H1E Antibody (N-term) (Cat. #AP13710a) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the HIST1H1E antibody detected the HIST1H1E protein (arrow).
  • IHC-P - HIST1H1E Antibody (N-term) AP13710a
    HIST1H1E Antibody (N-term) (Cat. #AP13710a)immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of HIST1H1E Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P10412
Other Accession P02252, NP_005312.1
Reactivity Human
Predicted Rabbit
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 21865 Da
Antigen Region 1-30 aa
Additional Information
Gene ID 3008
Other Names Histone H14, Histone H1b, Histone H1s-4, HIST1H1E, H1F4
Target/Specificity This HIST1H1E antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human HIST1H1E.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHIST1H1E Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms H1F4
Function Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation (By similarity).
Cellular Location Nucleus. Chromosome. Note=Mainly localizes in heterochromatin. Dysplays a punctuate staining pattern in the nucleus
Citations (0)

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Histones are basic nuclear proteins responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H1 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.


Trojer, P., et al. J. Biol. Chem. 284(13):8395-8405(2009)
Lee, C.Z., et al. Virology 375(1):197-204(2008)
Trojer, P., et al. Cell 129(5):915-928(2007)
Wu, C., et al. Proteomics 7(11):1775-1785(2007)
Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)

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Cat# AP13710a
(40 western blots)
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