|Application ||WB, IHC-P, E|
|Calculated MW||15145 Da|
|Antigen Region||111-140 aa|
|Other Names||Histone H2AX, H2a/x, Histone H2AX, H2AFX, H2AX|
|Target/Specificity||This H2AFX antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 111-140 amino acids from the C-terminal region of human H2AFX.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||H2AFX Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C- terminal phosphorylation.|
|Cellular Location||Nucleus. Chromosome.|
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Provided below are standard protocols that you may find useful for product applications.
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif.
Roch-Lefevre, S., et al. Radiat. Res. 174(2):185-194(2010)
Schmid, T.E., et al. Int. J. Radiat. Biol. 86(8):682-691(2010)
Jiang, X., et al. FEBS Lett. 584(13):2926-2930(2010)
Vasireddy, R.S., et al. Br. J. Cancer 102(10):1511-1518(2010)
Ikeda, M., et al. Int. J. Oncol. 36(5):1081-1088(2010)
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