|Application ||WB, E|
|Calculated MW||70255 Da|
|Antigen Region||412-440 aa|
|Other Names||Melanocyte protein PMEL, ME20-M, ME20M, Melanocyte protein Pmel 17, Melanocytes lineage-specific antigen GP100, Melanoma-associated ME20 antigen, P1, P100, Premelanosome protein, Silver locus protein homolog, M-alpha, 95 kDa melanocyte-specific secreted glycoprotein, P26, Secreted melanoma-associated ME20 antigen, ME20-S, ME20S, M-beta, PMEL, D12S53E, PMEL17, SILV|
|Target/Specificity||This SILV antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 412-440 amino acids from the Central region of human SILV.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||SILV Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||D12S53E, PMEL17, SILV|
|Function||Plays a central role in the biogenesis of melanosomes. Involved in the maturation of melanosomes from stage I to II. The transition from stage I melanosomes to stage II melanosomes involves an elongation of the vesicle, and the appearance within of distinct fibrillar structures. Release of the soluble form, ME20-S, could protect tumor cells from antibody mediated immunity.|
|Cellular Location||Endoplasmic reticulum membrane; Single-pass type I membrane protein. Golgi apparatus. Melanosome. Endosome, multivesicular body. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Localizes predominantly to intralumenal vesicles (ILVs) within multivesicular bodies. Associates with ILVs found within the lumen of premelanosomes and melanosomes and particularly in compartments that serve as precursors to the striated stage II premelanosomes|
|Tissue Location||Preferentially expressed in melanomas. Some expression was found in dysplastic nevi. Not found in normal tissues nor in carcinomas. Normally expressed at low levels in quiescent adult melanocytes but overexpressed by proliferating neonatal melanocytes and during tumor growth|
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Provided below are standard protocols that you may find useful for product applications.
SILV plays a central role in the biogenesis of melanosomes. Involved in the maturation of melanosomes from stage I to II. The transition from stage I melanosomes to stage II melanosomes involves an elongation of the vesicle, and the appearance within of distinct fibrillar structures. Release of the soluble form, ME20-S, could protect tumor cells from antibody mediated immunity.
Leonhardt, R.M., et al. J. Biol. Chem. 285(21):16166-16183(2010)
Hoashi, T., et al. FASEB J. 24(3):916-930(2010)
Watt, B., et al. J. Biol. Chem. 284(51):35543-35555(2009)
McGlinchey, R.P., et al. Proc. Natl. Acad. Sci. U.S.A. 106(33):13731-13736(2009)
Kummer, M.P., et al. J. Biol. Chem. 284(4):2296-2306(2009)
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