|Application ||WB, E|
|Other Accession||Q9Z2D8, NP_003917.1|
|Calculated MW||32844 Da|
|Antigen Region||1-30 aa|
|Other Names||Methyl-CpG-binding domain protein 3, Methyl-CpG-binding protein MBD3, MBD3|
|Target/Specificity||This MBD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human MBD3.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||MBD3 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Acts as transcriptional repressor and plays a role in gene silencing. Does not bind to DNA by itself (PubMed:12124384). Binds to DNA with a preference for sites containing methylated CpG dinucleotides (in vitro). Binds to a lesser degree DNA containing unmethylated CpG dinucleotides (PubMed:24307175). Recruits histone deacetylases and DNA methyltransferases.|
|Cellular Location||Nucleus. Chromosome. Note=Nuclear, in discrete foci. Detected on chromatin, at promoter regions of active genes|
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Provided below are standard protocols that you may find useful for product applications.
DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). However, unlike the other family members, MBD3 is not capable of binding to methylated DNA. The predicted MBD3 protein shares 71% and 94% identity with MBD2 (isoform 1) and mouse Mbd3. MBD3 is a subunit of the NuRD, a multisubunit complex containing nucleosome remodeling and histone deacetylase activities. MBD3 mediates the association of metastasis-associated protein 2 (MTA2) with the core histone deacetylase complex.
Bachmann, N., et al. Eur J Med Genet 53(1):23-24(2010)
Noh, E.J., et al. Biochem. Biophys. Res. Commun. 378(3):332-337(2009)
Morey, L., et al. Mol. Cell. Biol. 28(19):5912-5923(2008)
Brown, S.E., et al. Gene 420(2):99-106(2008)
Spensberger, D., et al. Biochemistry 47(24):6418-6426(2008)
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