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PRMT5 Antibody (N-term)Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AP13773a | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
| AP13773a-ev20 | Ev20 size 100 ul | In Stock | $ 95.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
- Reviews
- Protocols
- Backgrounds
PRMT5 Antibody (N-term) - Product info | |
| Application | WB, IHC
|
| Primary Accession | O14744 |
| Other Accession | Q4R5M3, A7YW45, NP_006100.2, NP_001034708.1 |
| Reactivity | Human |
| Predicted | Bovine, Monkey |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 72684 Da |
PRMT5 Antibody (N-term) - Additional info | |
| Gene ID 10419 | |
| Other Names PRMT5; HRMT1L5; IBP72; JBP1; SKB1; Protein arginine N-methyltransferase 5; 72 kDa ICln-binding protein; Histone-arginine N-methyltransferase PRMT5; Jak-binding protein 1; Shk1 kinase-binding protein 1 homolog; Protein arginine N-methyltransferase 5, N-terminally processed | |
| Target/Specificity This PRMT5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 75-104 amino acids from the N-terminal region of human PRMT5. | |
| Dilution WB~~1:100~500WB~~1:1000 IHC~~1:10~50 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions PRMT5 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. | |
PRMT5 Antibody (N-term) - Protein Information | |
| Name PRMT5 | |
| Synonyms HRMT1L5, IBP72, JBP1, SKB1 | |
| Function Arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and symmetrical dimethylarginine (sDMA), with a preference for the formation of MMA. Specifically mediates the symmetrical dimethylation of arginine residues in the small nuclear ribonucleoproteins Sm D1 (SNRPD1) and Sm D3 (SNRPD3); such methylation being required for the assembly and biogenesis of snRNP core particles. Methylates SUPT5H. Mono- and dimethylates arginine residues of myelin basic protein (MBP) in vitro. Plays a role in the assembly of snRNP core particles. May play a role in cytokine-activated transduction pathways. Negatively regulates cyclin E1 promoter activity and cellular proliferation. May regulate the SUPT5H transcriptional elongation properties. May be part of a pathway that is connected to a chloride current, possibly through cytoskeletal rearrangement. Methylates histone H2A and H4 'Arg-3' during germ cell development. Methylates histone H3 'Arg-8', which may repress transcription. Methylates the Piwi proteins (PIWIL1, PIWIL2 and PIWIL4), methylation of Piwi proteins being required for the interaction with Tudor domain-containing proteins and subsequent localization to the meiotic nuage. Methylates RPS10. Attenuates EGF signaling through the MAPK1/MAPK3 pathway acting at 2 levels First, monomethylates EGFR; this enhances EGFR 'Tyr-1197' phosphorylation and PTPN6 recruitment, eventually leading to reduced SOS1 phosphorylation. Second, methylates RAF1 and probably BRAF, hence destabilizing these 2 signaling proteins and reducing their catalytic activity. Required for induction of E-selectin and VCAM-1, on the endothelial cells surface at sites of inflammation Methylates HOXA9. Methylates and regulates SRGAP2 which is involved in cell migration and differentiation | |
| Cellular Location Cytoplasm. Nucleus. | |
| Tissue Location Ubiquitous. | |
PRMT5 Antibody (N-term) - Related products
AP1007a: PRMT5 Antibody (N-term)
AP1007b: PRMT5 Antibody (C-term C487)
AP1007d: PRMT5 Antibody (N-term P88)
AP1008a: PRMT5 Antibody (C-term)
AP13773a: PRMT5 Antibody (N-term)
RI14244: PRMT5 predesign siRNA
BP1007a: PRMT5 Antibody (N-term) Blocking Peptide
BP1007b: PRMT5 Antibody (C-term C487) Blocking Peptide
BP1007d: PRMT5 Antibody (N-term P88) Blocking Peptide
PRMT5 Antibody (N-term) - Application data
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PRMT5 Antibody (N-term) (Cat. #AP13773a) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the PRMT5 antibody detected the PRMT5 protein (arrow).
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PRMT5 Antibody (N-term) (Cat. #AP13773a)immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PRMT5 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
PRMT5 Antibody (N-term) - Research Areas
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BACKGROUND
Arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and symmetrical dimethylarginine (sDMA), with a preference for the formation of MMA. Specifically mediates the symmetrical dimethylation of arginine residues in the small nuclear ribonucleoproteins Sm D1 (SNRPD1) and Sm D3 (SNRPD3); such methylation being required for the assembly and biogenesis of snRNP core particles. Methylates SUPT5H. Mono-and dimethylates arginine residues of myelin basic protein (MBP) in vitro. Plays a role in the assembly of snRNP core particles. May play a role in cytokine-activated transduction pathways. Negatively regulates cyclin E1 promoter activity and cellular proliferation. May regulate the SUPT5H transcriptional elongation properties. May be part of a pathway that is connected to a chloride current, possibly through cytoskeletal rearrangement. Methylates histone H2A and H4 'Arg-3' during germ cell development. Methylates histone H3 'Arg-8', which may repress transcription. Methylates the Piwi proteins (PIWIL1, PIWIL2 and PIWIL4), methylation of Piwi proteins being required for the interaction with Tudor domain-containing proteins and subsequent localization to the meiotic nuage. Methylates RPS10.
REFERENCES
Aggarwal, P., et al. Cancer Cell 18(4):329-340(2010) Rank, G., et al. Blood 116(9):1585-1592(2010) Cesaro, E., et al. J. Biol. Chem. 284(47):32321-32330(2009) Zhao, Q., et al. Nat. Struct. Mol. Biol. 16(3):304-311(2009) Bruns, A.F., et al. Biol. Chem. 390(1):59-65(2009)
