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NR0B1 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - NR0B1 Antibody (Center) AP13774c
    NR0B1 Antibody (Center) (Cat. #AP13774c) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the NR0B1 antibody detected the NR0B1 protein (arrow).
  • WB - NR0B1 Antibody (Center) AP13774c
    Western blot analysis of NR0B1 (arrow) using rabbit polyclonal NR0B1 Antibody (Center) (Cat. #AP13774c). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the NR0B1 gene.
  • IHC-P - NR0B1 Antibody (Center) AP13774c
    NR0B1 Antibody (Center) (Cat. #AP13774c)immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NR0B1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P51843
Other Accession NP_000466.2
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 51718 Da
Antigen Region 304-333 aa
Additional Information
Gene ID 190
Other Names Nuclear receptor subfamily 0 group B member 1, DSS-AHC critical region on the X chromosome protein 1, Nuclear receptor DAX-1, NR0B1, AHC, DAX1
Target/Specificity This NR0B1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 304-333 amino acids from the Central region of human NR0B1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNR0B1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NR0B1
Synonyms AHC, DAX1
Function Orphan nuclear receptor. Component of a cascade required for the development of the hypothalamic-pituitary-adrenal-gonadal axis. Acts as a coregulatory protein that inhibits the transcriptional activity of other nuclear receptors through heterodimeric interactions. May also have a role in the development of the embryo and in the maintenance of embryonic stem cell pluripotency (By similarity).
Cellular Location Nucleus. Cytoplasm. Note=Shuttles between the cytoplasm and nucleus. Homodimers exits in the cytoplasm and in the nucleus
Research Areas
Citations (0)

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This gene encodes a protein that contains a DNA-binding domain. The encoded protein acts as a dominant-negative regulator of transcription which is mediated by the retinoic acid receptor. This protein also functions as an anti-testis gene by acting antagonistically to Sry. Mutations in this gene result in both X-linked congenital adrenal hypoplasia and hypogonadotropic hypogonadism.


Li, N., et al. J. Clin. Endocrinol. Metab. 95 (9), E104-E111 (2010) :
Nedumaran, B., et al. J. Biol. Chem. 285(12):9221-9232(2010)
Kinsey, M., et al. Cancer Res. 69(23):9047-9055(2009)
Nagl, F., et al. Am. J. Physiol., Cell Physiol. 297 (5), C1146-C1156 (2009) :
Skinningsrud, B., et al. J. Clin. Endocrinol. Metab. 94(10):4086-4093(2009)

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Cat# AP13774c
(40 western blots)
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