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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   Mouse Bckdk Antibody (Center)   

Mouse Bckdk Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - Mouse Bckdk Antibody (Center) AP13798c
    Mouse Bckdk Antibody (Center) (Cat. #AP13798c) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the Bckdk antibody detected the Bckdk protein (arrow).
  • WB - Mouse Bckdk Antibody (Center) AP13798c
    Mouse Bckdk Antibody (Center) (Cat. #AP13798c) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the Bckdk antibody detected the Bckdk protein (arrow).
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  • CITATIONS
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession O55028
Other Accession NP_033869.1
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 46588 Da
Antigen Region 159-188 aa
Additional Information
Gene ID 12041
Other Names [3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase, mitochondrial, Branched-chain alpha-ketoacid dehydrogenase kinase, BCKD-kinase, BCKDHKIN, Bckdk
Target/Specificity This Mouse Bckdk antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 159-188 amino acids from the Central region of mouse Bckdk.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMouse Bckdk Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Bckdk
Function Catalyzes the phosphorylation and inactivation of the branched-chain alpha-ketoacid dehydrogenase complex, the key regulatory enzyme of the valine, leucine and isoleucine catabolic pathways. Key enzyme that regulate the activity state of the BCKD complex.
Cellular Location Mitochondrion matrix. Mitochondrion {ECO:0000250|UniProtKB:O14874}
Tissue Location Ubiquitous.
Research Areas
Citations (0)

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Background

Catalyzes the phosphorylation and inactivation of the branched-chain alpha-ketoacid dehydrogenase complex, the key regulatory enzyme of the valine, leucine and isoleucine catabolic pathways. Key enzyme that regulate the activity state of the BCKD complex (By similarity).

References

Pagliarini, D.J., et al. Cell 134(1):112-123(2008)
Lee, J., et al. Mol. Cell Proteomics 6(4):669-676(2007)
Hutson, S.M. Biochem. J. 400 (1), E1-E3 (2006) :
Joshi, M.A., et al. Biochem. J. 400(1):153-162(2006)
Trinidad, J.C., et al. Mol. Cell Proteomics 5(5):914-922(2006)

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$ 295.00
$ 99.00
Cat# AP13798c
Size:
Quantity:
(40 western blots)
Availability: In Stock
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