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AP1G2 Antibody (N-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - AP1G2 Antibody (N-term) AP13809a
    AP1G2 Antibody (N-term) (Cat. #AP13809a) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the AP1G2 antibody detected the AP1G2 protein (arrow).
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession O75843
Other Accession NP_003908.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 87117 Da
Antigen Region 1-30 aa
Additional Information
Gene ID 8906
Other Names AP-1 complex subunit gamma-like 2, Gamma2-adaptin, G2ad, AP1G2
Target/Specificity This AP1G2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human AP1G2.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsAP1G2 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name AP1G2
Function May function in protein sorting in late endosomes or multivesucular bodies (MVBs).
Cellular Location Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side Cytoplasmic vesicle membrane; Peripheral membrane protein. Endosome membrane; Peripheral membrane protein. Note=Mainly localized to perinuclear vesicular structures (PubMed:9733768). Colocalizes with HBV major surface antigen L and HBV core protein C in CD63-containing compartments (PubMed:16867982). Colocalizes with HBV major surface antigen L to cis-Golgi-like structures (PubMed:11333915)
Tissue Location Expressed in all but one (skeletal muscle) tissues examined
Research Areas
Citations (0)
citation

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Background

Adaptins are important components of clathrin-coated vesicles transporting ligand-receptor complexes from the plasma membrane or from the trans-Golgi network to lysosomes. The adaptin family of proteins is compsed of four classes of molecules named alpha, beta-, beta prime- and gamma- adaptins. Adaptins, together with medium and small subunits, form a heterotetrameric complex called an adaptor, whose role is to promote the formation of clathrin-coated pits and vesicles. The protein encoded by this gene is a gamma-adaptin protein and it belongs to the adaptor complexes large subunits family. This protein along with the complex is thought to function at some trafficking step in the complex pathways between the trans-Golgi network and the cell surface. There are two alternatively spliced transcript variants of this gene encoding the same protein.

References

Doring, T., et al. Biochim. Biophys. Acta 1803(11):1252-1264(2010)
Rost, M., et al. J. Biol. Chem. 283(46):32119-32130(2008)
Lambert, C., et al. J. Virol. 81(17):9050-9060(2007)
Lehner, B., et al. Genome Res. 14(7):1315-1323(2004)
Mattera, R., et al. J. Biol. Chem. 279(9):8018-8028(2004)

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$ 365.00
$ 140.00
Cat# AP13809a
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