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EHD1 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - EHD1 Antibody (C-term) AP13814b
    All lanes : Anti-EHD1 Antibody (C-term) at 1:1000 dilution Lane 1: HepG2 whole cell lysate Lane 2: Hela whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 61kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - EHD1 Antibody (C-term) AP13814b
    EHD1 Antibody (C-term) (Cat. #AP13814b) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the EHD1 antibody detected the EHD1 protein (arrow).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9H4M9
Other Accession Q641Z6, Q9WVK4, Q5E9R3, NP_006786.2
Reactivity Human
Predicted Bovine, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 60627 Da
Antigen Region 410-438 aa
Additional Information
Gene ID 10938
Other Names EH domain-containing protein 1, PAST homolog 1, hPAST1, Testilin, EHD1, PAST, PAST1
Target/Specificity This EHD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 410-438 amino acids from the C-terminal region of human EHD1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsEHD1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name EHD1
Synonyms PAST, PAST1
Function Acts in early endocytic membrane fusion and membrane trafficking of recycling endosomes. Plays a role in myoblast fusion. Recruited to endosomal membranes upon nerve growth factor stimulation, indirectly regulates neurite outgrowth.
Cellular Location Recycling endosome membrane; Peripheral membrane protein. Early endosome membrane; Peripheral membrane protein. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Note=Preferentially associates with tubular recycling endosomes. Colocalizes with FER1L5 at plasma membrane in myoblasts and myotubes
Tissue Location Highly expressed in testis.
Research Areas
Citations (0)

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This gene belongs to a highly conserved gene family encoding EPS15 homology (EH) domain-containing proteins. The protein-binding EH domain was first noted in EPS15, a substrate for the epidermal growth factor receptor. The EH domain has been shown to be an important motif in proteins involved in protein-protein interactions and in intracellular sorting. The protein encoded by this gene is thought to play a role in the endocytosis of IGF1 receptors.


Gudmundsson, H., et al. Circ. Res. 107(1):84-95(2010)
Sharma, M., et al. Mol. Biol. Cell 20(24):5181-5194(2009)
Kieken, F., et al. Protein Sci. 18(12):2471-2479(2009)
Jovic, M., et al. Mol. Biol. Cell 20(11):2731-2743(2009)
Fichtman, B., et al. Cell. Mol. Biol. Lett. 13(4):632-648(2008)

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$ 295.00
$ 99.00
Cat# AP13814b
(40 western blots)
Availability: In Stock
Bulk Size
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