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POLR3B Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - POLR3B Antibody (N-term) AP13819A
    POLR3B Antibody (N-term) (Cat. #AP13819a) western blot analysis in mouse spleen tissue lysates (35ug/lane).This demonstrates the POLR3B antibody detected the POLR3B protein (arrow).
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q9NW08
Other Accession P59470, NP_060552.4
Reactivity Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 127785 Da
Antigen Region 330-359 aa
Additional Information
Gene ID 55703
Other Names DNA-directed RNA polymerase III subunit RPC2, RNA polymerase III subunit C2, C128, DNA-directed RNA polymerase III 1276 kDa polypeptide, DNA-directed RNA polymerase III subunit B, POLR3B
Target/Specificity This POLR3B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 330-359 amino acids from the N-terminal region of human POLR3B.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPOLR3B Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name POLR3B
Function DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol III is composed of mobile elements and RPC2 is part of the core element with the central large cleft and probably a clamp element that moves to open and close the cleft (By similarity). Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway.
Cellular Location Nucleus.
Citations (0)

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Background

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol III is composed of mobile elements and RPC2 is part of the core element with the central large cleft and probably a clamp element that moves to open and close the cleft (By similarity). Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF-Kappa-B through the RIG-I pathway.

References

Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Ablasser, A., et al. Nat. Immunol. 10(10):1065-1072(2009)
Shen, E., et al. Exp. Cell Res. 315(7):1100-1116(2009)
Yee, N.S., et al. PLoS Biol. 5 (11), E312 (2007) :
Seshadri, S., et al. BMC Med. Genet. 8 SUPPL 1, S15 (2007) :

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$ 295.00
$ 99.00
Cat# AP13819A
Size:
Quantity:
(40 western blots)
Availability: In Stock
Bulk Size
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