|Application ||WB, E|
|Other Accession||Q8R565, P49449, NP_001800.1, NP_001035891.1|
|Calculated MW||15991 Da|
|Antigen Region||82-110 aa|
|Other Names||Histone H3-like centromeric protein A, Centromere autoantigen A, Centromere protein A, CENP-A, CENPA|
|Target/Specificity||This CENPA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 82-110 amino acids from the Central region of human CENPA.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||CENPA Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Histone H3-like variant which exclusively replaces conventional H3 in the nucleosome core of centromeric chromatin at the inner plate of the kinetochore. Required for recruitment and assembly of kinetochore proteins, mitotic progression and chromosome segregation. May serve as an epigenetic mark that propagates centromere identity through replication and cell division. The CENPA-H4 heterotetramer can bind DNA by itself (in vitro).|
|Cellular Location||Nucleus. Chromosome, centromere, kinetochore. Note=Localizes exclusively in the kinetochore domain of centromeres. Occupies a compact domain at the inner kinetochore plate stretching across 2 thirds of the length of the constriction but encompassing only one third of the constriction width and height|
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Provided below are standard protocols that you may find useful for product applications.
Centromeres are the differentiated chromosomal domains that specify the mitotic behavior of chromosomes. CENPA encodes a centromere protein which contains a histone H3 related histone fold domain that is required for targeting to the centromere. CENPA is proposed to be a component of a modified nucleosome or nucleosome-like structure in which it replaces 1 or both copies of conventional histone H3 in the (H3-H4)2 tetrameric core of the nucleosome particle. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq].
Sekulic, N., et al. Nature 467(7313):347-351(2010)
Carroll, C.W., et al. J. Cell Biol. 189(7):1143-1155(2010)
Maehara, K., et al. Mol. Cell. Biol. 30(9):2090-2104(2010)
Buscaino, A., et al. Curr. Opin. Genet. Dev. 20(2):118-126(2010)
Pironon, N., et al. BMC Genomics 11, 195 (2010) :
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