|Application ||WB, IHC-P, E|
|Calculated MW||143908 Da|
|Antigen Region||737-765 aa|
|Other Names||Structural maintenance of chromosomes protein 1B, SMC protein 1B, SMC-1-beta, SMC-1B, SMC1B, SMC1L2|
|Target/Specificity||This SMC1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 737-765 amino acids from the Central region of human SMC1B.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||SMC1B Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Meiosis-specific component of cohesin complex. Required for the maintenance of meiotic cohesion, but not, or only to a minor extent, for its establishment. Contributes to axial element (AE) formation and the organization of chromatin loops along the AE. Plays a key role in synapsis, recombination and chromosome movements. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The meiosis-specific cohesin complex probably replaces mitosis specific cohesin complex when it dissociates from chromatin during prophase I (By similarity).|
|Cellular Location||Nucleus. Chromosome. Chromosome, centromere. Note=Associates with chromatin. In prophase I stage of meiosis, localizes along the AE of synaptonemal complexes. In late-pachytene-diplotene, the bulk of protein dissociates from the chromosome arms probably because of phosphorylation by PLK, except at centromeres, where cohesin complexes remain. Remains chromatin associated at the centromeres up to metaphase II. At anaphase II, dissociates from centromeres, allowing chromosomes segregation (By similarity).|
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Provided below are standard protocols that you may find useful for product applications.
SMC1L2 belongs to a family of proteins required for chromatid cohesion and DNA recombination during meiosis and mitosis (3:Revenkova et al., 2001 [PubMed 11564881]).
Cobbe, N., et al. Mol. Biol. Evol. 21(2):332-347(2004) Revenkova, E., et al. Mol. Cell. Biol. 21(20):6984-6998(2001) Harrington, J.J., et al. Nat. Biotechnol. 19(5):440-445(2001)
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