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CAPN11 Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - CAPN11 Antibody (Center) AP13870c
    CAPN11 Antibody (Center) (Cat. #AP13870c) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the CAPN11 antibody detected the CAPN11 protein (arrow).
    detail
  • IHC-P - CAPN11 Antibody (Center) AP13870c
    CAPN11 Antibody (Center) (AP13870c)immunohistochemistry analysis in formalin fixed and paraffin embedded human testis carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CAPN11 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-P, WB, E
Primary Accession Q9UMQ6
Other Accession NP_008989.2
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 84423 Da
Antigen Region 409-438 aa
Additional Information
Gene ID 11131
Other Names Calpain-11, 3422-, Calcium-activated neutral proteinase 11, CANP 11, CAPN11
Target/Specificity This CAPN11 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 409-438 amino acids from the Central region of human CAPN11.
Dilution WB~~1:1000
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCAPN11 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CAPN11
Function Calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.
Cellular Location Cytoplasmic vesicle, secretory vesicle, acrosome
Tissue Location Highest expression in testis.
Research Areas
Citations (0)
citation

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Background

Calpains constitute a family of intracellular calcium-dependent cysteine proteases. There are eight members in this superfamily. They consist of a variable 80 kDa subunit and an invariant 30 kDa subunit. This calpain protein appears to have protease activity and calcium-binding ability. A similar mouse protein may play a functional role in spermatogenesis and in the regulation of calcium-dependent signal transduction events during meiosis.

References

Ben-Aharon, I., et al. Mol. Reprod. Dev. 73(6):767-773(2006)
Dear, T.N., et al. Genomics 59(2):243-247(1999)
Rojas, F.J., et al. Mol. Hum. Reprod. 5(6):520-526(1999)
Saido, T.C., et al. FASEB J. 8(11):814-822(1994)
Melloni, E., et al. Trends Neurosci. 12(11):438-444(1989)

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$ 365.00
$ 140.00
Cat# AP13870c
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