|Application ||WB, E|
|Other Accession||Q9QZ67, NP_003611.1|
|Calculated MW||66675 Da|
|Antigen Region||183-212 aa|
|Other Names||Protein phosphatase 1D, Protein phosphatase 2C isoform delta, PP2C-delta, Protein phosphatase magnesium-dependent 1 delta, p53-induced protein phosphatase 1, PPM1D, WIP1|
|Target/Specificity||This PPM1D antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 183-212 amino acids from the Central region of human PPM1D.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||PPM1D Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Required for the relief of p53-dependent checkpoint mediated cell cycle arrest. Binds to and dephosphorylates 'Ser-15' of TP53 and 'Ser-345' of CHEK1 which contributes to the functional inactivation of these proteins. Mediates MAPK14 dephosphorylation and inactivation (PubMed:21283629).|
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Provided below are standard protocols that you may find useful for product applications.
The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. The expression of this gene is induced in a p53-dependent manner in response to various environmental stresses. While being induced by tumor suppressor protein TP53/p53, this phosphatase negatively regulates the activity of p38 MAP kinase, MAPK/p38, through which it reduces the phosphorylation of p53, and in turn suppresses p53-mediated transcription and apoptosis. This phosphatase thus mediates a feedback regulation of p38-p53 signaling that contributes to growth inhibition and the suppression of stress induced apoptosis. This gene is located in a chromosomal region known to be amplified in breast cancer. The amplification of this gene has been detected in both breast cancer cell line and primary breast tumors, which suggests a role of this gene in cancer development.
Zhang, X., et al. Cancer Res. 70(18):7176-7186(2010)
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Moon, S.H., et al. J. Biol. Chem. 285(17):12935-12947(2010)
Macurek, L., et al. Oncogene 29(15):2281-2291(2010)
Yang, D.H., et al. Zhonghua Yi Xue Za Zhi 90(8):519-522(2010)
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