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DNASE1L3 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - DNASE1L3 Antibody (N-term) AP13885a
    DNASE1L3 Antibody (N-term) (Cat. #AP13885a) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the DNASE1L3 antibody detected the DNASE1L3 protein (arrow).
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q13609
Other Accession NP_004935.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 35504 Da
Antigen Region 14-43 aa
Additional Information
Gene ID 1776
Other Names Deoxyribonuclease gamma, DNase gamma, 3121-, DNase I homolog protein DHP2, Deoxyribonuclease I-like 3, DNase I-like 3, Liver and spleen DNase, LS-DNase, LSD, DNASE1L3, DHP2, DNAS1L3
Target/Specificity This DNASE1L3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 14-43 amino acids from the N-terminal region of human DNASE1L3.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsDNASE1L3 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name DNASE1L3
Synonyms DHP2, DNAS1L3
Function Has DNA hydrolytic activity. Is capable of both single- and double-stranded DNA cleavage, producing DNA fragments with 3'- OH ends (By similarity). Can cleave chromatin to nucleosomal units and cleaves nucleosomal and liposome-coated DNA (PubMed:9070308, PubMed:9714828, PubMed:14646506, PubMed:10807908, PubMed:27293190). Acts in internucleosomal DNA fragmentation (INDF) during apoptosis and necrosis (PubMed:23229555, PubMed:24312463). The role in apoptosis includes myogenic and neuronal differentiation, and BCR-mediated clonal deletion of self-reactive B cells (By similarity). Is active on chromatin in apoptotic cell-derived membrane-coated microparticles and thus suppresses anti-DNA autoimmunity (PubMed:27293190).
Cellular Location Nucleus. Endoplasmic reticulum. Secreted. Note=Translocates from the endoplasmic reticulum to the nucleus during apoptosis (PubMed:23229555). Contradictory reports exist about the subcellular localization under normal physiological conditions Under conditions of cell death, may diffuse and/or be actively transported to the nucleus. {ECO:0000269|PubMed:23229555, ECO:0000305}
Tissue Location Liver and spleen.
Research Areas
Citations (0)

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Background

This gene encodes a member of the DNase family. The protein hydrolyzes DNA, is not inhibited by actin, and mediates the breakdown of DNA during apoptosis. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized.

References

Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Ueki, M., et al. Clin. Chim. Acta 407 (1-2), 20-24 (2009) :
Mizuta, R., et al. Biomed. Res. 30(3):165-170(2009)
Boulares, H., et al. Biochem. Biophys. Res. Commun. 341(2):653-662(2006)
Okamoto, M., et al. Biochem. Biophys. Res. Commun. 327(1):76-83(2005)

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Cat# AP13885a
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(40 western blots)
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