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CDK9 Antibody (C-term)Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AP13892b | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
| AP13892b-ev20 | 20 ug 100 ul | In Stock | $ 95.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
- Reviews
- Protocols
- Backgrounds
CDK9 Antibody (C-term) - Product info | |
| Application | WB, IF
|
| Primary Accession | P50750 |
| Other Accession | Q641Z4, Q99J95, Q5EAB2, NP_001252.1 |
| Reactivity | Human |
| Predicted | Bovine, Mouse, Rat |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 42778 Da |
CDK9 Antibody (C-term) - Additional info | |
| Gene ID 1025 | |
| Other Names CDK9; CDC2L4; TAK; Cyclin-dependent kinase 9; C-2K; Cell division cycle 2-like protein kinase 4; Cell division protein kinase 9; Serine/threonine-protein kinase PITALRE; Tat-associated kinase complex catalytic subunit | |
| Target/Specificity This CDK9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 252-282 amino acids from the C-terminal region of human CDK9. | |
| Dilution WB~~1:100~500WB~~1:1000 IF~~1:10~50 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions CDK9 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. | |
CDK9 Antibody (C-term) - Protein Information | |
| Name CDK9 | |
| Synonyms CDC2L4, TAK | |
| Function Protein kinase involved in the regulation of transcription. Member of the cyclin-dependent kinase pair (CDK9/cyclin-T) complex, also called positive transcription elongation factor b (P-TEFb), which facilitates the transition from abortive to productive elongation by phosphorylating the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAP II) POLR2A, SUPT5H and RDBP. This complex is inactive when in the 7SK snRNP complex form. Phosphorylates EP300, MYOD1, RPB1/POLR2A and AR, and the negative elongation factors DSIF and NELF. Regulates cytokine inducible transcription networks by facilitating promoter recognition of target transcription factors (e.g. TNF-inducible RELA/p65 activation and IL-6-inducible STAT3 signaling). Promotes RNA synthesis in genetic programs for cell growth, differentiation and viral pathogenesis. P-TEFb is also involved in cotranscriptional histone modification, mRNA processing and mRNA export. Modulates a complex network of chromatin modifications including histone H2B monoubiquitination (H2Bub1), H3 lysine 4 trimethylation (H3K4me3) and H3K36me3; integrates phosphorylation during transcription with chromatin modifications to control co-transcriptional histone mRNA processing. The CDK9/cyclin-K complex has also a kinase activity towards CTD of RNAP II and can substitute for CDK9/cyclin-T P-TEFb in vitro. Replication stress response protein; the CDK9/cyclin-K complex is required for genome integrity maintenance, by promoting cell cycle recovery from replication arrest and limiting single- stranded DNA amount in response to replication stress, thus reducing the breakdown of stalled replication forks and avoiding DNA damage. In addition, probable function in DNA repair of isoform 2 via interaction with KU70/XRCC6. Promotes cardiac myocyte enlargement. RPB1/POLR2A phosphorylation on 'Ser-2' in CTD activates transcription. AR phosphorylation modulates AR transcription factor promoter selectivity and cell growth. DSIF and NELF phosphorylation promotes transcription by inhibiting their negative effect. The phosphorylation of MYOD1 enhances its transcriptional activity and thus promotes muscle differentiation | |
| Cellular Location Nucleus. Cytoplasm. Nucleus, PML body. Note=Accumulates on chromatin in response to replication stress Complexed with CCNT1 in nuclear speckles, but uncomplexed form in the cytoplasm. The translocation from nucleus to cytoplasm is XPO1/CRM1-dependent. Associates with PML body when acetylated | |
| Tissue Location Ubiquitous. | |
CDK9 Antibody (C-term) - Related products
AP13892b: CDK9 Antibody (C-term)
AP7525b: CDK9 Antibody (C-term)
LY10679a: CDK9 Over-expression Lysate
CDK9 Antibody (C-term) - Application data
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CDK9 Antibody (C-term) (Cat. #AP13892b) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the CDK9 antibody detected the CDK9 protein (arrow).
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Confocal immunofluorescent analysis of CDK9 Antibody (C-term) (Cat. #AP13892b) with 293 cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
CDK9 Antibody (C-term) - Research Areas
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BACKGROUND
The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2, and known as important cell cycle regulators. This kinase was found to be a component of the multiprotein complex TAK/P-TEFb, which is an elongation factor for RNA polymerase II-directed transcription and functions by phosphorylating the C-terminal domain of the largest subunit of RNA polymerase II. This protein forms a complex with and is regulated by its regulatory subunit cyclin T or cyclin K. HIV-1 Tat protein was found to interact with this protein and cyclin T, which suggested a possible involvement of this protein in AIDS.
REFERENCES
Dow, E.C., et al. J. Cell. Physiol. 224(1):84-93(2010) Liu, H., et al. Biochem. Biophys. Res. Commun. 397(2):245-250(2010) Pirngruber, J., et al. Oncogene 29(19):2853-2863(2010) Belakavadi, M., et al. Mol. Cell. Biol. 30(10):2437-2448(2010) Khiati, A., et al. J. Neurovirol. 16(2):150-167(2010)
