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OCLN Antibody (C-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - OCLN Antibody (C-term) AP13901b
    Western blot analysis of lysate from MCF-7 cell line, using OCLN Antibody (C-term)(Cat. #AP13901b). AP13901b was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q16625
Other Accession Q61146, NP_002529.1
Reactivity Human
Predicted Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 59144 Da
Antigen Region 446-474 aa
Additional Information
Gene ID 100506658
Other Names Occludin, OCLN
Target/Specificity This OCLN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 446-474 amino acids from the C-terminal region of human OCLN.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsOCLN Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name OCLN
Function May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. It is able to induce adhesion when expressed in cells lacking tight junctions.
Cellular Location Cell membrane; Multi-pass membrane protein. Cell junction, tight junction
Tissue Location Localized at tight junctions of both epithelial and endothelial cells. Highly expressed in kidney. Not detected in testis
Research Areas
Citations (0)
citation

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Background

This gene encodes an integral membrane protein which is located at tight junctions. This protein may be involved in the formation and maintenance of the tight junction. The possibility of several alternatively spliced products has been suggested but the full nature of these products has not been described. [provided by RefSeq].

References

Liu, S., et al. Virology 407(1):160-170(2010)
Michta, M.L., et al. J. Virol. 84(22):11696-11708(2010)
O'Driscoll, M.C., et al. Am. J. Hum. Genet. 87(3):354-364(2010)
Van Itallie, C.M., et al. J. Cell. Sci. 123 (PT 16), 2844-2852 (2010) :
Raikwar, N.S., et al. Am. J. Physiol. Renal Physiol. 299 (2), F436-F444 (2010) :

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$ 182.50
$ 70.00
Cat# AP13901b
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