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Mouse Eif2ak1 Antibody (N-term)Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AP13913a | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
| AP13913a-ev20 | 20 ug 100 ul | In Stock | $ 95.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
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Mouse Eif2ak1 Antibody (N-term) - Product info | |
| Application | WB, IHC
|
| Primary Accession | Q9Z2R9 |
| Other Accession | Q63185, P33279 |
| Reactivity | Human, Mouse |
| Predicted | Rat, Rabbit |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 69702 Da |
Mouse Eif2ak1 Antibody (N-term) - Additional info | |
| Gene ID 15467 | |
| Other Names Eif2ak1; Hri; Eukaryotic translation initiation factor 2-alpha kinase 1; Heme-controlled repressor; Heme-regulated eukaryotic initiation factor eIF-2-alpha kinase; Heme-regulated inhibitor; Hemin-sensitive initiation factor 2-alpha kinase | |
| Target/Specificity This Mouse Eif2ak1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 15-44 amino acids from the N-terminal region of Mouse Eif2ak1. | |
| Dilution WB~~1:100~500WB~~1:1000 IHC~~1:10~50 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions Mouse Eif2ak1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. | |
Mouse Eif2ak1 Antibody (N-term) - Protein Information | |
| Name Eif2ak1 | |
| Synonyms Hri | |
| Function Inhibits protein synthesis at the translation initiation level, in response to various stress conditions, including oxidative stress, heme deficiency, osmotic shock and heat shock Exerts its function through the phosphorylation of EIF2S1 at 'Ser- 48' and 'Ser-51', thus preventing its recycling. Binds hemin forming a 1:1 complex through a cysteine thiolate and histidine nitrogenous coordination. This binding occurs with moderate affinity, allowing it to sense the heme concentration within the cell. Thanks to this unique heme-sensing capacity, plays a crucial role to shut off protein synthesis during acute heme-deficient conditions. In red blood cells (RBCs), controls hemoglobin synthesis ensuring a coordinated regulation of the synthesis of its heme and globin moieties. Thus plays an essential protective role for RBC survival in anemias of iron deficiency. Similarly, in hepatocytes, involved in heme-mediated translational control of CYP2B and CYP3A and possibly other hepatic P450 cytochromes. May also contain ER stress during acute heme-deficient conditions | |
| Cellular Location Cytoplasm. | |
| Tissue Location Expressed predominantly in erythroid cells, mature reticulocytes, as well as fetal liver nucleated erythroid cells. At much lower levels, expressed in hepatocytes and bone marrow-derived macrophages (at protein level) | |
Mouse Eif2ak1 Antibody (N-term) - Related products
Mouse Eif2ak1 Antibody (N-term) - Application data
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Mouse Eif2ak1 Antibody (N-term) (Cat. #AP13913a) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the Eif2ak1 antibody detected the Eif2ak1 protein (arrow).
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Mouse Eif2ak1 Antibody (N-term) (Cat. #AP13913a) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the Eif2ak1 antibody detected the Eif2ak1 protein (arrow).
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Mouse Eif2ak1 Antibody (N-term) (AP13913a)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Eif2ak1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Mouse Eif2ak1 Antibody (N-term) - Research Areas
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BACKGROUND
Mediates down-regulation of protein synthesis in response to various stress conditions by the phosphorylation of EIF2S1 at 'Ser-48' and 'Ser-51'. Protein synthesis is inhibited at the level of initiation.
