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Mouse Nlk Antibody (C-term)Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AP13929b | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
| AP13929b-ev20 | 20 ug 100 ul | In Stock | $ 95.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
- Reviews
- Protocols
- Backgrounds
Mouse Nlk Antibody (C-term) - Product info | |
| Application | WB, IHC
|
| Primary Accession | O54949 |
| Other Accession | D3ZSZ3, Q9UBE8, E1BMN8, NP_032728.3, XP_001472539.1 |
| Reactivity | Human, Mouse |
| Predicted | Bovine, Rat |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 58313 Da |
Mouse Nlk Antibody (C-term) - Additional info | |
| Gene ID 18099 | |
| Other Names Nlk; Serine/threonine-protein kinase NLK; Nemo-like kinase | |
| Target/Specificity This Mouse Nlk antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 407-436 amino acids from the C-terminal region of human Mouse Nlk. | |
| Dilution WB~~1:100~500WB~~1:1000 IHC~~1:10~50 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions Mouse Nlk Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. | |
Mouse Nlk Antibody (C-term) - Protein Information | |
| Name Nlk | |
| Function Serine/threonine-protein kinase that regulates a number of transcription factors with key roles in cell fate determination. Positive effector of the non-canonical Wnt signaling pathway, acting downstream of WNT5A, MAP3K7/TAK1 and HIPK2. Activation of this pathway causes binding to and phosphorylation of the histone methyltransferase SETDB1. The NLK- SETDB1 complex subsequently interacts with PPARG, leading to methylation of PPARG target promoters at histone H3K9 and transcriptional silencing. The resulting loss of PPARG target gene transcription inhibits adipogenesis and promotes osteoblastogenesis in mesenchymal stem cells (MSCs). Negative regulator of the canonical Wnt/beta-catenin signaling pathway Binds to and phosphorylates TCF7L2/TCF4 and LEF1, promoting the dissociation of the TCF7L2/LEF1/beta-catenin complex from DNA, as well as the ubiquitination and subsequent proteolysis of LEF1 Together these effects inhibit the transcriptional activation of canonical Wnt/beta-catenin target genes. Negative regulator of the Notch signaling pathway. Binds to and phosphorylates NOTCH1, thereby preventing the formation of a transcriptionally active ternary complex of NOTCH1, RBPJ/RBPSUH and MAML1. Negative regulator of the MYB family of transcription factors Phosphorylation of MYB leads to its subsequent proteolysis while phosphorylation of MYBL1 and MYBL2 inhibits their interaction with the coactivator CREBBP. Other transcription factors may also be inhibited by direct phosphorylation of CREBBP itself. Acts downstream of IL6 and MAP3K7/TAK1 to phosphorylate STAT3, which is in turn required for activation of NLK by MAP3K7/TAK1 | |
| Cellular Location Nucleus. Cytoplasm. Note=Predominantly nuclear. A smaller fraction is cytoplasmic | |
| Tissue Location Expressed at high levels in the brain, and at lower levels in heart, kidney, lung and liver | |
Mouse Nlk Antibody (C-term) - Related products
Mouse Nlk Antibody (C-term) - Application data
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Mouse Nlk Antibody (C-term) (Cat. #AP13929b) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the Nlk antibody detected the Nlk protein (arrow).
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Mouse Nlk Antibody (C-term) (Cat. #AP13929b) western blot analysis in mouse lung tissue lysates (35ug/lane).This demonstrates the Nlk antibody detected the Nlk protein (arrow).
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Mouse Nlk Antibody (C-term) (AP13929b)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Nlk Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Mouse Nlk Antibody (C-term) - Research Areas
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BACKGROUND
Role in cell fate determination, required for differentiation of bone marrow stromal cells. Acts downstream of MAP3K7 and HIPK2 to negatively regulate the canonical Wnt/beta-catenin signaling pathway and the phosphorylation and destruction of the MYB transcription factor. May suppress a wide range of transcription factors by phosphorylation of the coactivator, CREBBP. Involved in TGFbeta-mediated mesoderm induction, acting downstream of MAP3K7/TAK1 to phosphorylate STAT3.
REFERENCES
Li, M., et al. J. Biol. Chem. 285(18):13397-13404(2010) Nifuji, A., et al. Exp. Cell Res. 316(7):1127-1136(2010) Ishitani, T., et al. Nat. Cell Biol. 12(3):278-285(2010) Martinez, G., et al. Invest. Ophthalmol. Vis. Sci. 50(10):4794-4806(2009) Harwood, B.N., et al. Dev. Dyn. 237(4):1099-1111(2008)
