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CHST14 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - CHST14 Antibody (Center) AP13951c
    CHST14 Antibody (Center) (Cat. #AP13951c) western blot analysis in mouse stomach tissue lysates (15ug/lane).This demonstrates the CHST14 antibody detected CHST14 protein (arrow).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q8NCH0
Other Accession Q80V53, NP_569735.1
Reactivity Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 42997 Da
Antigen Region 178-205 aa
Additional Information
Gene ID 113189
Other Names Carbohydrate sulfotransferase 14, Dermatan 4-sulfotransferase 1, D4ST-1, hD4ST1, CHST14, D4ST1
Target/Specificity This CHST14 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 178-205 amino acids from the Central region of human CHST14.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCHST14 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CHST14
Synonyms D4ST1
Function Catalyzes the transfer of sulfate to position 4 of the N-acetylgalactosamine (GalNAc) residue of dermatan sulfate. Plays a pivotal role in the formation of 4-0-sulfated IdoA blocks in dermatan sulfate. Transfers sulfate to the C-4 hydroxyl of beta1,4-linked GalNAc that is substituted with an alpha-linked iduronic acid (IdoUA) at the C-3 hydroxyl. Transfers sulfate more efficiently to GalNAc residues in -IdoUA-GalNAc-IdoUA- than in -GlcUA-GalNAc-GlcUA-sequences. Has preference for partially desulfated dermatan sulfate. Addition of sulfate to GalNAc may occur immediately after epimerization of GlcUA to IdoUA. GlcUA to IdoUA. Appears to have an important role in the formation of the cerbellar neural network during postnatal brain development.
Cellular Location Golgi apparatus membrane; Single-pass type II membrane protein
Tissue Location Widely expressed. Expressed at high level in pituitary gland, placenta, uterus and thyroid
Citations (0)

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This gene encodes a member of the HNK-1 family of sulfotransferases. The encoded protein transfers sulfate to the C-4 hydroxyl of N-acetylgalactosamine residues in dermatan sulfate. Mutations in this gene have been associated with adducted thumb-clubfoot syndrome.


Miyake, N., et al. Hum. Mutat. 31(8):966-974(2010)
Dundar, M., et al. Am. J. Hum. Genet. 85(6):873-882(2009)
Pacheco, B., et al. Glycobiology 19(11):1197-1203(2009)
Lamesch, P., et al. Genomics 89(3):307-315(2007)
Mikami, T., et al. J. Biol. Chem. 278(38):36115-36127(2003)

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Cat# AP13951c
(40 western blots)
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