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ERG25 Antibody (C-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| IHC-P, WB, E |
|---|---|
| Primary Accession | Q15800 |
| Other Accession | NP_006736.1, NP_001017369.1 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 35216 Da |
| Antigen Region | 264-293 aa |
| Gene ID | 6307 |
|---|---|
| Other Names | Methylsterol monooxygenase 1, C-4 methylsterol oxidase, MSMO1, DESP4, ERG25, SC4MOL |
| Target/Specificity | This ERG25 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 264-293 amino acids from the C-terminal region of human ERG25. |
| Dilution | WB~~1:1000 IHC-P~~1:10~50 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | ERG25 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | MSMO1 |
|---|---|
| Synonyms | DESP4, ERG25, SC4MOL |
| Function | Catalyzes the three-step monooxygenation required for the demethylation of 4,4-dimethyl and 4alpha-methylsterols, which can be subsequently metabolized to cholesterol (PubMed:21285510, PubMed:28673550, PubMed:23583456, PubMed:26114596). Also involved in drug metabolism, as it can metabolize eldecalcitol (ED-71 or 1alpha,25- dihydroxy-2beta-(3-hydroxypropoxy)-cholecalciferol), a second- generation vitamin D analog, into 1alpha,2beta,25-trihydroxy vitamin D3; this reaction occurs via enzymatic hydroxylation and spontaneous O- dehydroxypropylation (PubMed:26038696). |
| Cellular Location | Endoplasmic reticulum membrane; Multi-pass membrane protein |
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Provided below are standard protocols that you may find useful for product applications.
Background
Sterol-C4-mehtyl oxidase-like protein was isolated based on its similarity to the yeast ERG25 protein. It contains a set of putative metal binding motifs with similarity to that seen in a family of membrane desaturases-hydroxylases. The protein is localized to the endoplasmic reticulum membrane and is believed to function in cholesterol biosynthesis. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
References
Lu, Y., et al. J. Lipid Res. 49(12):2582-2589(2008)
Lim, J., et al. Cell 125(4):801-814(2006)
Li, L., et al. J. Biol. Chem. 271(28):16927-16933(1996)
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