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NOX5 Antibody (C-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - NOX5 Antibody (C-term) AP13968b
    NOX5 Antibody (C-term) (Cat. #AP13968b) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the NOX5 antibody detected the NOX5 protein (arrow).
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q96PH1
Other Accession NP_001171708.1, NP_078781.3, NP_001171709.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 86439 Da
Antigen Region 680-709 aa
Additional Information
Gene ID 79400
Other Names NADPH oxidase 5, 163-, NOX5
Target/Specificity This NOX5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 680-709 amino acids from the C-terminal region of human NOX5.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNOX5 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NOX5
Function Calcium-dependent NADPH oxidase that catalyzes the generation of superoxide from molecular oxygen utilizing NADPH as an electron donor (PubMed:12686516). May play a role in cell growth and apoptosis (PubMed:12686516).
Cellular Location [Isoform v2]: Endoplasmic reticulum. Cell membrane; Multi-pass membrane protein. Note=Calcium-sensitive association and dissociation between the N- and C-terminal domains appears to facilitate its localization to the cell membrane
Tissue Location Mainly expressed in pachytene spermatocytes of testis and in lymphocyte-rich areas of spleen and lymph nodes. Also detected in ovary, placenta, pancreas, cardiac fibroblasts. Expressed in B-cells and prostate malignant cells. [Isoform v2]: Expressed in microvascular endothelial cells (at protein level) (PubMed:17275676). Expressed in testis (PubMed:11483596). Expressed in endothelial cells and pulmonary artery smooth muscle cells (PubMed:17275676, PubMed:22427510) [Isoform v4]: Expressed in endothelial cells and pulmonary artery smooth muscle cells.
Research Areas
Citations (0)
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Background

Calcium-dependent NADPH oxidase that generates superoxide. Also functions as a calcium-dependent proton channel and may regulate redox-dependent processes in lymphocytes and spermatozoa. May play a role in cell growth and apoptosis.

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$ 365.00
$ 140.00
Cat# AP13968b
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