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PNPLA4 Antibody (C-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - PNPLA4 Antibody (C-term) AP13971b
    PNPLA4 Antibody (C-term) (Cat. #AP13971b) western blot analysis in WiDr cell line lysates (35ug/lane).This demonstrates the PNPLA4 antibody detected the PNPLA4 protein (arrow).
    detail
  • IHC-P - PNPLA4 Antibody (C-term) AP13971b
    PNPLA4 Antibody (C-term) (Cat. #AP13971b)immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PNPLA4 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession P41247
Other Accession NP_004641.1, NP_001135861.1, NP_001166143.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 27980 Da
Antigen Region 171-199 aa
Additional Information
Gene ID 8228
Other Names Patatin-like phospholipase domain-containing protein 4, Protein GS2, PNPLA4, DXS1283E, GS2
Target/Specificity This PNPLA4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 171-199 amino acids from the C-terminal region of human PNPLA4.
Dilution WB~~1:1000
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPNPLA4 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PNPLA4 (HGNC:24887)
Function Has abundant triacylglycerol lipase activity (PubMed:15364929, PubMed:16150821, PubMed:17603008). Transfers fatty acid from triglyceride to retinol, hydrolyzes retinylesters, and generates 1,3-diacylglycerol from triglycerides (PubMed:17603008). Additionally possesses acylglycerol transacylase and phospholipase A2 activities (PubMed:15364929, PubMed:17603008).
Cellular Location Mitochondrion.
Tissue Location Expressed in all tissues examined, including heart, brain, placenta, lung, liver, muscle, kidney, pancreas and spleen
Research Areas
Citations (0)
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Background

This gene encodes a member of the patatin-like family of phospholipases. The encoded enzyme has both triacylglycerol lipase and transacylase activities and may be involved in adipocyte triglyceride homeostasis. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome Y.

References

Gao, J.G., et al. Mol. Genet. Metab. 96(4):253-260(2009)
Kienesberger, P.C., et al. J. Lipid Res. 50 SUPPL, S63-S68 (2009) :
Johansson, L.E., et al. PLoS ONE 4 (4), E5327 (2009) :
Gao, J.G., et al. Biochem. Biophys. Res. Commun. 360(2):501-506(2007)
Wilson, P.A., et al. J. Lipid Res. 47(9):1940-1949(2006)

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$ 182.50
$ 70.00
Cat# AP13971b
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