|Application ||WB, FC, IHC-P, E|
|Other Accession||NP_003191.1, NP_001129611.1|
|Calculated MW||67600 Da|
|Antigen Region||506-534 aa|
|Other Names||Transcription factor E2-alpha, Class B basic helix-loop-helix protein 21, bHLHb21, Immunoglobulin enhancer-binding factor E12/E47, Immunoglobulin transcription factor 1, Kappa-E2-binding factor, Transcription factor 3, TCF-3, Transcription factor ITF-1, TCF3, BHLHB21, E2A, ITF1|
|Target/Specificity||This TCF3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 506-534 amino acids from the C-terminal region of human TCF3.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||TCF3 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||BHLHB21, E2A, ITF1|
|Function||Transcriptional regulator. Involved in the initiation of neuronal differentiation. Heterodimers between TCF3 and tissue- specific basic helix-loop-helix (bHLH) proteins play major roles in determining tissue-specific cell fate during embryogenesis, like muscle or early B-cell differentiation. Dimers bind DNA on E- box motifs: 5'-CANNTG-3'. Binds to the kappa-E2 site in the kappa immunoglobulin gene enhancer. Binds to IEB1 and IEB2, which are short DNA sequences in the insulin gene transcription control region.|
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Provided below are standard protocols that you may find useful for product applications.
The TCF3 gene, also called E2A, encodes 2 basic helix-loop-helix (bHLH) transcription factors, E12 and E47, through alternative splicing. E12 and E47 are involved in regulation of immunoglobulin gene expression (Bain et al., 1994 [PubMed 8001125]).
Hirose, K., et al. Blood 116(6):962-970(2010)
Pan, F., et al. Immunogenetics 62(4):237-251(2010)
Hauser, J., et al. Mol. Immunol. 47(5):1031-1038(2010)
Rittie, L., et al. Aging Cell 8(6):738-751(2009)
Pedraza, N., et al. J. Biol. Chem. 284(47):32980-32988(2009)
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