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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   TCF3 Antibody (C-term)   

TCF3 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - TCF3 Antibody (C-term) AP13991b
    All lanes : Anti-TCF3 Antibody (C-term) at 1:1000 dilution Lane 1: Jurkat whole cell lysate Lane 2: Ramos whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 68 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - TCF3 Antibody (C-term) AP13991b
    TCF3 Antibody (C-term) (Cat. #AP13991b) western blot analysis in human uterine tumor tissue lysates (35ug/lane).This demonstrates the TCF3 antibody detected the TCF3 protein (arrow).
  • WB - TCF3 Antibody (C-term) AP13991b
    TCF3 Antibody (C-term) (Cat. #AP13991b) western blot analysis in mouse cerebellum tissue lysates (35ug/lane).This demonstrates the TCF3 antibody detected the TCF3 protein (arrow).
  • FC - TCF3 Antibody (C-term) AP13991b
    TCF3 Antibody (C-term) (Cat. #AP13991b) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
  • IHC-P - TCF3 Antibody (C-term) AP13991b
    TCF3 Antibody (C-term) (Cat. #AP13991b)immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TCF3 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P15923
Other Accession NP_003191.1, NP_001129611.1
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 67600 Da
Antigen Region 506-534 aa
Additional Information
Gene ID 6929
Other Names Transcription factor E2-alpha, Class B basic helix-loop-helix protein 21, bHLHb21, Immunoglobulin enhancer-binding factor E12/E47, Immunoglobulin transcription factor 1, Kappa-E2-binding factor, Transcription factor 3, TCF-3, Transcription factor ITF-1, TCF3, BHLHB21, E2A, ITF1
Target/Specificity This TCF3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 506-534 amino acids from the C-terminal region of human TCF3.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTCF3 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TCF3
Synonyms BHLHB21, E2A, ITF1
Function Transcriptional regulator. Involved in the initiation of neuronal differentiation. Heterodimers between TCF3 and tissue- specific basic helix-loop-helix (bHLH) proteins play major roles in determining tissue-specific cell fate during embryogenesis, like muscle or early B-cell differentiation. Dimers bind DNA on E- box motifs: 5'-CANNTG-3'. Binds to the kappa-E2 site in the kappa immunoglobulin gene enhancer. Binds to IEB1 and IEB2, which are short DNA sequences in the insulin gene transcription control region.
Cellular Location Nucleus.
Research Areas
Citations (0)

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The TCF3 gene, also called E2A, encodes 2 basic helix-loop-helix (bHLH) transcription factors, E12 and E47, through alternative splicing. E12 and E47 are involved in regulation of immunoglobulin gene expression (Bain et al., 1994 [PubMed 8001125]).


Hirose, K., et al. Blood 116(6):962-970(2010)
Pan, F., et al. Immunogenetics 62(4):237-251(2010)
Hauser, J., et al. Mol. Immunol. 47(5):1031-1038(2010)
Rittie, L., et al. Aging Cell 8(6):738-751(2009)
Pedraza, N., et al. J. Biol. Chem. 284(47):32980-32988(2009)

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$ 295.00
$ 99.00
Cat# AP13991b
(40 western blots)
Availability: In Stock
Bulk Size
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