TCF3 Antibody (C-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS: 1
- PROTOCOLS
- BACKGROUND
Application
| WB, FC, IHC-P, E |
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Primary Accession | P15923 |
Other Accession | NP_003191.1, NP_001129611.1 |
Reactivity | Human, Mouse |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 67600 Da |
Antigen Region | 506-534 aa |
Gene ID | 6929 |
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Other Names | Transcription factor E2-alpha, Class B basic helix-loop-helix protein 21, bHLHb21, Immunoglobulin enhancer-binding factor E12/E47, Immunoglobulin transcription factor 1, Kappa-E2-binding factor, Transcription factor 3, TCF-3, Transcription factor ITF-1, TCF3, BHLHB21, E2A, ITF1 |
Target/Specificity | This TCF3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 506-534 amino acids from the C-terminal region of human TCF3. |
Dilution | WB~~1:1000 IHC-P~~1:10~50 FC~~1:10~50 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | TCF3 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | TCF3 |
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Synonyms | BHLHB21, E2A, ITF1 |
Function | Transcriptional regulator involved in the initiation of neuronal differentiation and mesenchymal to epithelial transition (By similarity). Heterodimers between TCF3 and tissue-specific basic helix- loop-helix (bHLH) proteins play major roles in determining tissue- specific cell fate during embryogenesis, like muscle or early B-cell differentiation (By similarity). Together with TCF15, required for the mesenchymal to epithelial transition (By similarity). Dimers bind DNA on E-box motifs: 5'-CANNTG-3' (By similarity). Binds to the kappa-E2 site in the kappa immunoglobulin gene enhancer (PubMed:2493990). Binds to IEB1 and IEB2, which are short DNA sequences in the insulin gene transcription control region (By similarity). |
Cellular Location | Nucleus. |
Provided below are standard protocols that you may find useful for product applications.
Background
The TCF3 gene, also called E2A, encodes 2 basic helix-loop-helix (bHLH) transcription factors, E12 and E47, through alternative splicing. E12 and E47 are involved in regulation of immunoglobulin gene expression (Bain et al., 1994 [PubMed 8001125]).
References
Hirose, K., et al. Blood 116(6):962-970(2010)
Pan, F., et al. Immunogenetics 62(4):237-251(2010)
Hauser, J., et al. Mol. Immunol. 47(5):1031-1038(2010)
Rittie, L., et al. Aging Cell 8(6):738-751(2009)
Pedraza, N., et al. J. Biol. Chem. 284(47):32980-32988(2009)
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