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BRD1 Antibody (N-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - BRD1 Antibody (N-term) AP14018a
    BRD1 Antibody (N-term) (Cat. #AP14018a) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the BRD1 antibody detected the BRD1 protein (arrow).
    detail
  • IHC-P - BRD1 Antibody (N-term) AP14018a
    BRD1 Antibody (N-term) (AP14018a)immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of BRD1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession O95696
Other Accession NP_055392.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 119520 Da
Antigen Region 73-102 aa
Additional Information
Gene ID 23774
Other Names Bromodomain-containing protein 1, BR140-like protein, Bromodomain and PHD finger-containing protein 2, BRD1, BRL, BRPF2
Target/Specificity This BRD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 73-102 amino acids from the N-terminal region of human BRD1.
Dilution WB~~1:1000
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsBRD1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name BRD1 {ECO:0000303|PubMed:16387653, ECO:0000312|HGNC:HGNC:1102}
Function Scaffold subunit of various histone acetyltransferase (HAT) complexes, such as the MOZ/MORF and HBO1 complexes, that acts as a regulator of hematopoiesis (PubMed:16387653, PubMed:21753189, PubMed:21880731). Plays a key role in HBO1 complex by directing KAT7/HBO1 specificity towards histone H3 'Lys-14' acetylation (H3K14ac), thereby promoting erythroid differentiation (PubMed:21753189).
Cellular Location Nucleus. Chromosome Note=Localizes to transcription start sites
Tissue Location Highly expressed in testis.
Citations (0)
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Background

This gene encodes a protein of unknown function. The protein contains a bromodomain, a sequence motif often found in transcriptional coactivators, and localizes to the nucleus in testis and several other cell types.

References

Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Kushima, I., et al. Am. J. Med. Genet. B Neuropsychiatr. Genet. 153B (3), 786-791 (2010) :
Nyegaard, M., et al. Am. J. Med. Genet. B Neuropsychiatr. Genet. 153B (2), 582-591 (2010) :
Bjarkam, C.R., et al. Brain Struct Funct 214(1):37-47(2009)
Olsen, J.V., et al. Cell 127(3):635-648(2006)

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$ 182.50
$ 70.00
Cat# AP14018a
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