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CDH4 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - CDH4 Antibody (N-term) AP1401A
    All lanes : Anti-CDH4 Antibody (N-term) at 1:2000 dilution Lane 1: A431 whole cell lysate Lane 2: SH-SY5Y whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 100 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - CDH4 Antibody (N-term) AP1401A
    Anti-CDH4 Antibody (N-term) at 1:2000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 100 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • IHC-P - CDH4 Antibody (N-term) AP1401A
    Formalin-fixed and paraffin-embedded human brain tissue reacted with CDH4 antibody (N-term) (Cat.#AP1401a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • IF - CDH4 Antibody (N-term) AP1401A
    Immunofluorescence analysis of CDH4 Antibody (N-term) Antibody with paraffin-embedded human brain tissue . 0.025 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.Red counterstaining is PI.
  • FC - CDH4 Antibody (N-term) AP1401A
    Flow cytometric analysis of HepG2 cells using CDH4 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P55283
Other Accession Q63149, P39038
Reactivity Human
Predicted Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 100281 Da
Antigen Region 175-203 aa
Additional Information
Gene ID 1002
Other Names Cadherin-4, Retinal cadherin, R-CAD, R-cadherin, CDH4
Target/Specificity This CDH4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 175-203 amino acids from the N-terminal region of human CDH4.
Dilution WB~~1:2000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCDH4 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CDH4
Function Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. May play an important role in retinal development.
Cellular Location Cell membrane; Single-pass type I membrane protein
Tissue Location Expressed mainly in brain but also found in other tissues
Research Areas
Citations ( 0 )


CDH4 is a classical cadherin from the cadherin superfamily. It is a calcium-dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Based on studies in chicken and mouse, this cadherin is thought to play an important role during brain segmentation and neuronal outgrowth. In addition, a role in kidney and muscle development is indicated. Of particular interest are studies showing stable cis-heterodimers of cadherins 2 and 4 in cotransfected cell lines. Previously thought to interact in an exclusively homophilic manner, this is the first evidence of cadherin heterodimerization.


Miotto,E., Cancer Res. 64 (22), 8156-8159 (2004)
Johnson,E., J. Biol. Chem. 279 (30), 31041-31049 (2004)
Kitagawa,M., Biochem. Biophys. Res. Commun. 271 (2), 358-363 (2000)

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$ 295.00
$ 99.00
Cat# AP1401A
(40 western blots)
Availability: In Stock
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