- Antibodies
- New
- Biological Process >
- Cellular Compartment >
- Disease >
- Molecular Function >
- Pathway Biocarta >
- Pathway KEGG >
- Pathway Panther >
- Tissue >
- Tag
- Peptides
- Biological Process >
- Cellular Compartment >
- Disease >
- Molecular Function >
- Pathway Biocarta >
- Pathway KEGG >
- Pathway Panther >
- Tissue >
- Amino Acids
- Tag
- Biological Process >
- Proteins
- RNAi
- FL cDNA Clones
- Cell/Tissues/Lysates
CDH4 Antibody (N-term)Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
Australia Austria Belgium Brazil Bulgaria Canada China Croatia Cyprus Czech Republic Denmark Estonia Finland France Germany Greece Hungary Iceland India Indonesia Ireland Israel Italy Japan Korea Latvia Lithuania Luxembourg Macedonia Malaysia Malta Netherlands Norway Pakistan Poland Portugal Romania Serbia Singapore Slovakia Slovenia Spain Sweden Switzerland Taiwan Turkey United Kingdom United States Vietnam Others 
|
|||
|---|---|---|---|---|
| Catalog # | Size | Availability | Price | |
| AP1401a | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
| AP1401a-ev20 | 20 ug 100 ul | In Stock | $ 95.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
- Reviews
- Protocols
- Backgrounds
CDH4 Antibody (N-term) - Product info | |
| Application | WB, IHC, IF, FC
|
| Primary Accession | P55283 |
| Other Accession | Q63149, P39038 |
| Reactivity | Human |
| Predicted | Mouse, Rat |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 100281 Da |
CDH4 Antibody (N-term) - Additional info | |
| Gene ID 1002 | |
| Other Names CDH4; Cadherin-4; Retinal cadherin | |
| Target/Specificity This CDH4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 182~211 amino acids from the N-terminal region of human CDH4. | |
| Dilution WB~~1:50~100WB~~1:1000 IHC~~1:10~50 IF~~1:10~50 FC~~1:10~50 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. Then the antibody is purified by peptide affinity purification. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions CDH4 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. | |
CDH4 Antibody (N-term) - Protein Information | |
| Name CDH4 | |
| Function Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. May play an important role in retinal development | |
| Cellular Location Cell membrane; Single-pass type I membrane protein | |
| Tissue Location Expressed mainly in brain but also found in other tissues | |
CDH4 Antibody (N-term) - Related products
CDH4 Antibody (N-term) - Application data
-
Western blot analysis of anti-CDH4 Antibody (N-term) (RB13654) in HepG2 cell line lysates (35ug/lane). CDH4(arrow) was detected using the purified Pab (1:60 dilution).
-
Formalin-fixed and paraffin-embedded human brain tissue reacted with CDH4 antibody (N-term) (Cat.#AP1401a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
-
Immunofluorescence analysis of CDH4 Antibody (N-term) Antibody with paraffin-embedded human brain tissue . 0.025 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.Red counterstaining is PI.
-
Flow cytometric analysis of HepG2 cells using CDH4 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
CDH4 Antibody (N-term) - Research Areas
Abgent welcomes feedback from its customers.
If you have used an Abgent product and would like to share how it has performed, please click on the
"Submit Review" button and provide the requested information. Our staff will examine and post your
review and contact you if needed.
If you have any additional inquiries please email technical services at tech@abgent.com.
Thank you for your support.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
CDH4 is a classical cadherin from the cadherin superfamily. It is a calcium-dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Based on studies in chicken and mouse, this cadherin is thought to play an important role during brain segmentation and neuronal outgrowth. In addition, a role in kidney and muscle development is indicated. Of particular interest are studies showing stable cis-heterodimers of cadherins 2 and 4 in cotransfected cell lines. Previously thought to interact in an exclusively homophilic manner, this is the first evidence of cadherin heterodimerization.
REFERENCES
Miotto,E., Cancer Res. 64 (22), 8156-8159 (2004) Johnson,E., J. Biol. Chem. 279 (30), 31041-31049 (2004) Kitagawa,M., Biochem. Biophys. Res. Commun. 271 (2), 358-363 (2000)
