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MCM3 Antibody (N-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | P25205 |
| Other Accession | P25206, A4FUD9, NP_002379.2 |
| Reactivity | Human |
| Predicted | Bovine, Mouse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 90981 Da |
| Antigen Region | 36-64 aa |
| Gene ID | 4172 |
|---|---|
| Other Names | DNA replication licensing factor MCM3, DNA polymerase alpha holoenzyme-associated protein P1, P1-MCM3, RLF subunit beta, p102, MCM3 |
| Target/Specificity | This MCM3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 36-64 amino acids from the N-terminal region of human MCM3. |
| Dilution | WB~~1:1000 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | MCM3 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | MCM3 (HGNC:6945) |
|---|---|
| Function | Acts as a component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built (PubMed:32453425, PubMed:34694004, PubMed:34700328, PubMed:35585232). The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity (PubMed:32453425). Required for the entry in S phase and for cell division (Probable). |
| Cellular Location | Nucleus. Chromosome. Note=Associated with chromatin before the formation of nuclei and detaches from it as DNA replication progresses. |
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Background
The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with, and thus is acetlyated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression. [provided by RefSeq].
References
Lau, K.M., et al. Oncogene 29(40):5475-5489(2010)
Olson, J.E., et al. Breast Cancer Res. Treat. (2010) In press :
Lee, Y.S., et al. Exp. Mol. Pathol. 88(1):138-142(2010)
Song, Y.J., et al. Acta Virol. 54(2):125-130(2010)
Saade, E., et al. Proteomics 9(21):4934-4943(2009)
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