- CITATIONS: 1
|Application ||WB, IHC-P, E|
|Calculated MW||107895 Da|
|Antigen Region||932-960 aa|
|Other Names||General vesicular transport factor p115, Protein USO1 homolog, Transcytosis-associated protein, TAP, Vesicle-docking protein, USO1, VDP|
|Target/Specificity||This USO1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 932-960 amino acids from the C-terminal region of human USO1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||USO1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||General vesicular transport factor required for intercisternal transport in the Golgi stack; it is required for transcytotic fusion and/or subsequent binding of the vesicles to the target membrane. May well act as a vesicular anchor by interacting with the target membrane and holding the vesicular and target membranes in proximity (By similarity).|
|Cellular Location||Cytoplasm, cytosol. Golgi apparatus membrane; Peripheral membrane protein. Note=Recycles between the cytosol and the Golgi apparatus during interphase. During interphase, the phosphorylated form is found exclusively in cytosol; the unphosphorylated form is associated with Golgi apparatus membranes|
Provided below are standard protocols that you may find useful for product applications.
The protein encoded by this gene is a peripheral membrane protein which recycles between the cytosol and the Golgi apparatus during interphase. It is regulated by phosphorylation: dephosphorylated protein associates with the Golgi membrane and dissociates from the membrane upon phosphorylation. Ras-associated protein 1 recruits this protein to coat protein complex II (COPII) vesicles during budding from the endoplasmic reticulum, where it interacts with a set of COPII vesicle-associated SNAREs to form a cis-SNARE complex that promotes targeting to the Golgi apparatus. Transport from the ER to the cis/medial Golgi compartments requires the action of this gene product, GM130 and giantin in a sequential manner.
Striegl, H., et al. PLoS ONE 5 (2), E8991 (2010) :
Merk, M., et al. J. Immunol. 182(11):6896-6906(2009)
Mukherjee, S., et al. J. Biol. Chem. 284(3):1709-1717(2009)
Striegl, H., et al. PLoS ONE 4 (2), E4656 (2009) :
Guo, Y., et al. Mol. Biol. Cell 19(7):2830-2843(2008)
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at email@example.com.