|Application ||WB, IHC-P, E|
|Other Accession||Q9R1T1, O54962, P61283, NP_001137457.1, NP_003851.1|
|Predicted||Bovine, Mouse, Rat|
|Calculated MW||10059 Da|
|Antigen Region||37-65 aa|
|Other Names||Barrier-to-autointegration factor, Breakpoint cluster region protein 1, Barrier-to-autointegration factor, N-terminally processed, BANF1, BAF, BCRG1|
|Target/Specificity||This BANF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 37-65 amino acids from the Central region of human BANF1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||BANF1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Plays fundamental roles in nuclear assembly, chromatin organization, gene expression and gonad development. May potently compress chromatin structure and be involved in membrane recruitment and chromatin decondensation during nuclear assembly. Contains 2 non-specific dsDNA-binding sites which may promote DNA cross-bridging. Exploited by retroviruses for inhibiting self- destructing autointegration of retroviral DNA, thereby promoting integration of viral DNA into the host chromosome. EMD and BAF are cooperative cofactors of HIV-1 infection. Association of EMD with the viral DNA requires the presence of BAF and viral integrase. The association of viral DNA with chromatin requires the presence of BAF and EMD.|
|Cellular Location||Nucleus. Cytoplasm. Chromosome. Note=Significantly enriched at the nuclear inner membrane, diffusely throughout the nucleus during interphase and concentrated at the chromosomes during the M-phase May be included in HIV-1 virions via its interaction with viral GAG polyprotein. The phosphorylated form (by VRK1) shows a cytoplasmic localization|
|Tissue Location||Widely expressed. Expressed in colon, brain, heart, kidney, liver, lung, ovary, pancreas, placenta, prostate, skeletal muscle, small intestine, spleen and testis. Not detected in thymus and peripheral blood leukocytes|
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Provided below are standard protocols that you may find useful for product applications.
The protein encoded by this gene was first identified by its ability to protect retroviruses from intramolecular integration and therefore promote intermolecular integration into the host cell genome. The protein forms a homodimer which localizes to both the nucleus and cytoplasm and is specifically associated with chromosomes during mitosis. This protein binds to double stranded DNA in a non-specific manner and also binds to LEM-domain containing proteins of the nuclear envelope. This protein is thought to facilitate nuclear reassembly by binding with both DNA and inner nuclear membrane proteins and thereby recruit chromatin to the nuclear periphery. Alternative splicing results in multiple transcript variants encoding the same protein.
Montes de Oca, R., et al. PLoS ONE 4 (9), E7050 (2009) :
Haraguchi, T., et al. J. Cell. Sci. 121 (PT 15), 2540-2554 (2008) :
Haraguchi, T., et al. J. Cell. Sci. 120 (PT 12), 1967-1977 (2007) :
Wiebe, M.S., et al. Cell Host Microbe 1(3):187-197(2007)
Bengtsson, L., et al. Mol. Biol. Cell 17(3):1154-1163(2006)
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