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BANF1 Antibody (Center)Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AP14071c | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
| AP14071c-ev20 | 20 ug 100 ul | In Stock | $ 95.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
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BANF1 Antibody (Center) - Product info | |
| Application | WB, IHC
|
| Primary Accession | O75531 |
| Other Accession | Q9R1T1, O54962, P61283, NP_001137457.1, NP_003851.1 |
| Reactivity | Human |
| Predicted | Bovine, Mouse, Rat |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 10059 Da |
BANF1 Antibody (Center) - Additional info | |
| Gene ID 8815 | |
| Other Names BANF1; BAF; BCRG1; Barrier-to-autointegration factor; Breakpoint cluster region protein 1 | |
| Target/Specificity This BANF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 36-65 amino acids from the Central region of human BANF1. | |
| Dilution WB~~1:100~500WB~~1:1000 IHC~~1:10~50 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions BANF1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. | |
BANF1 Antibody (Center) - Protein Information | |
| Name BANF1 | |
| Synonyms BAF, BCRG1 | |
| Function Plays fundamental roles in nuclear assembly, chromatin organization, gene expression and gonad development. May potently compress chromatin structure and be involved in membrane recruitment and chromatin decondensation during nuclear assembly Contains 2 non-specific dsDNA-binding sites which may promote DNA cross-bridging. Exploited by retroviruses for inhibiting self- destructing autointegration of retroviral DNA, thereby promoting integration of viral DNA into the host chromosome. EMD and BAF are cooperative cofactors of HIV-1 infection. Association of EMD with the viral DNA requires the presence of BAF and viral integrase The association of viral DNA with chromatin requires the presence of BAF and EMD | |
| Cellular Location Nucleus. Cytoplasm. Chromosome. Note=Significantly enriched at the nuclear inner membrane, diffusely throughout the nucleus during interphase and concentrated at the chromosomes during the M-phase. May be included in HIV-1 virions via its interaction with viral GAG polyprotein. The phosphorylated form (by VRK1) shows a cytoplasmic localization | |
| Tissue Location Widely expressed. Expressed in colon, brain, heart, kidney, liver, lung, ovary, pancreas, placenta, prostate, skeletal muscle, small intestine, spleen and testis. Not detected in thymus and peripheral blood leukocytes | |
BANF1 Antibody (Center) - Related products
AP14071c: BANF1 Antibody (Center)
RI10536: BANF1 predesign siRNA
DC01821: Human BANF1 cDNA Clone
LY13504a: BANF1 Over-expression Lysate
BANF1 Antibody (Center) - Application data
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BANF1 Antibody (Center) (Cat. #AP14071c) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the BANF1 antibody detected the BANF1 protein (arrow).
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BANF1 Antibody (Center) (AP14071c)immunohistochemistry analysis in formalin fixed and paraffin embedded human colon tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of BANF1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
BANF1 Antibody (Center) - Research Areas
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BACKGROUND
The protein encoded by this gene was first identified by its ability to protect retroviruses from intramolecular integration and therefore promote intermolecular integration into the host cell genome. The protein forms a homodimer which localizes to both the nucleus and cytoplasm and is specifically associated with chromosomes during mitosis. This protein binds to double stranded DNA in a non-specific manner and also binds to LEM-domain containing proteins of the nuclear envelope. This protein is thought to facilitate nuclear reassembly by binding with both DNA and inner nuclear membrane proteins and thereby recruit chromatin to the nuclear periphery. Alternative splicing results in multiple transcript variants encoding the same protein.
REFERENCES
Montes de Oca, R., et al. PLoS ONE 4 (9), E7050 (2009) : Haraguchi, T., et al. J. Cell. Sci. 121 (PT 15), 2540-2554 (2008) : Haraguchi, T., et al. J. Cell. Sci. 120 (PT 12), 1967-1977 (2007) : Wiebe, M.S., et al. Cell Host Microbe 1(3):187-197(2007) Bengtsson, L., et al. Mol. Biol. Cell 17(3):1154-1163(2006)
