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>   home   >   Products   >   Primary Antibodies   >   Mouse Pkmyt1 Antibody (N-term)   

Mouse Pkmyt1 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - Mouse Pkmyt1 Antibody (N-term) AP14078a
    Mouse Pkmyt1 Antibody (N-term) (Cat. #AP14078a) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the Pkmyt1 antibody detected the Pkmyt1 protein (arrow).
  • WB - Mouse Pkmyt1 Antibody (N-term) AP14078a
    Western blot analysis of Pkmyt1 (arrow) using rabbit polyclonal Mouse Pkmyt1 Antibody (N-term) (Cat. #AP14078a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the Pkmyt1 gene.
  • WB - Mouse Pkmyt1 Antibody (N-term) AP14078a
    Mouse Pkmyt1 Antibody (N-term) (Cat. #AP14078a) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the Pkmyt1 antibody detected the Pkmyt1 protein (arrow).
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q9ESG9
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 54053 Da
Antigen Region 114-143 aa
Additional Information
Gene ID 268930
Other Names Membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase, Myt1 kinase, Pkmyt1, Myt1
Target/Specificity This Mouse Pkmyt1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 114-143 amino acids from the N-terminal region of mouse Pkmyt1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMouse Pkmyt1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Pkmyt1
Synonyms Myt1
Function Acts as a negative regulator of entry into mitosis (G2 to M transition) by phosphorylation of the CDK1 kinase specifically when CDK1 is complexed to cyclins. Mediates phosphorylation of CDK1 predominantly on 'Thr-14'. Also involved in Golgi fragmentation. May be involved in phosphorylation of CDK1 on 'Tyr-15' to a lesser degree, however tyrosine kinase activity is unclear and may be indirect. May be a downstream target of Notch signaling pathway during eye development (By similarity).
Cellular Location Endoplasmic reticulum membrane; Peripheral membrane protein. Golgi apparatus membrane; Peripheral membrane protein
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Background

Pkmyt1 acts as a negative regulator of entry into mitosis (G2 to M transition) by phosphorylation of the CDK1 kinase specifically when CDK1 is complexed to cyclins. Mediates phosphorylation of CDK1 predominantly on 'Thr-14'. Also involved in Golgi fragmentation. May be involved in phosphorylation of CDK1 on 'Tyr-15' to a lesser degree, however tyrosine kinase activity is unclear and may be indirect. May be a downstream target of Notch signaling pathway during eye development (By similarity).

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$ 295.00
$ 99.00
Cat# AP14078a
Size:
Quantity:
(40 western blots)
Availability: In Stock
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