|Application ||WB, E|
|Other Accession||Q9R012, NP_690017.2|
|Calculated MW||77812 Da|
|Antigen Region||354-382 aa|
|Other Names||Serine/threonine-protein kinase PLK2, Polo-like kinase 2, PLK-2, Serine/threonine-protein kinase SNK, Serum-inducible kinase, Plk2, Snk|
|Target/Specificity||This Mouse Plk2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 354-382 amino acids from the Central region of mouse Plk2.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Mouse Plk2 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Tumor suppressor serine/threonine-protein kinase involved in synaptic plasticity, centriole duplication and G1/S phase transition. Polo-like kinases act by binding and phosphorylating proteins are that already phosphorylated on a specific motif recognized by the POLO box domains. Phosphorylates CENPJ, NPM1, RAPGEF2, RASGRF1, SNCA, SIPA1L1 and SYNGAP1. Plays a key role in synaptic plasticity and memory by regulating the Ras and Rap protein signaling: required for overactivity-dependent spine remodeling by phosphorylating the Ras activator RASGRF1 and the Rap inhibitor SIPA1L1 leading to their degradation by the proteasome. Conversely, phosphorylates the Rap activator RAPGEF2 and the Ras inhibitor SYNGAP1, promoting their activity. Also regulates synaptic plasticity independently of kinase activity, via its interaction with NSF that disrupts the interaction between NSF and the GRIA2 subunit of AMPARs, leading to a rapid rundown of AMPAR-mediated current that occludes long term depression. Required for procentriole formation and centriole duplication by phosphorylating CENPJ and NPM1, respectively. Its induction by p53/TP53 suggests that it may participate in the mitotic checkpoint following stress.|
|Cellular Location||Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole. Cell projection, dendrite. Note=Localizes to centrosomes during early G1 phase where it only associates to the mother centriole and then distributes equally to both mother and daughter centrioles at the onset of S phase.|
|Tissue Location||Brain, lung and heart.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Plk2 may play a role in the division of at least some cell types, such as fibroblasts, and could function in embryogenesis, wound healing or neoplasia.
Hutchins, J.R., et al. Science 328(5978):593-599(2010)
Inglis, K.J., et al. J. Biol. Chem. 284(5):2598-2602(2009)
Park, Y.Y., et al. Biochem. Biophys. Res. Commun. 362(1):107-113(2007)
Nishimura, M., et al. DNA Res. 11(5):315-323(2004)
Ma, S., et al. Mol. Cell. Biol. 23(19):6936-6943(2003)
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