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EFCB7 Antibody (C-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - EFCB7 Antibody (C-term) AP14097b
    EFCB7 Antibody (C-term) (Cat. #AP14097b) western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).This demonstrates the EFCB7 antibody detected the EFCB7 protein (arrow).
    detail
  • IHC-P - EFCB7 Antibody (C-term) AP14097b
    EFCB7 Antibody (C-term) (Cat. #AP14097b)immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of EFCB7 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
    detail
  • FC - EFCB7 Antibody (C-term) AP14097b
    EFCB7 Antibody (C-term) (Cat. #AP14097b) flow cytometric analysis of Neuro-2a cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, FC, E
Primary Accession A8K855
Other Accession NP_115813.2
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 71981 Da
Antigen Region 434-462 aa
Additional Information
Gene ID 84455
Other Names EF-hand calcium-binding domain-containing protein 7, EFCAB7, KIAA1799
Target/Specificity This EFCB7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 434-462 amino acids from the C-terminal region of human EFCB7.
Dilution WB~~1:1000
IHC-P~~1:10~50
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsEFCB7 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name EFCAB7
Synonyms KIAA1799
Function Component of the EvC complex that positively regulates ciliary Hedgehog (Hh) signaling. Required for the localization of the EVC2:EVC subcomplex at the base of primary cilia.
Cellular Location Cell projection, cilium membrane {ECO:0000250|UniProtKB:Q8VDY4}; Peripheral membrane protein {ECO:0000250|UniProtKB:Q8VDY4}; Cytoplasmic side {ECO:0000250|UniProtKB:Q8VDY4}. Note=The EvC complex localizes at the base of cilia in the EvC zone of primary cilia in a EFCAB7-dependent manner. {ECO:0000250|UniProtKB:Q8VDY4}
Research Areas
Citations (0)
citation

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References

Mehrle, A., et al. Nucleic Acids Res. 34 (DATABASE ISSUE), D415-D418 (2006) :
Wiemann, S., et al. Genome Res. 14 (10B), 2136-2144 (2004) :

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$ 365.00
$ 140.00
Cat# AP14097b
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