|Application ||WB, IHC-P, E|
|Other Accession||NP_055232.1, NP_001120714.1, NP_001120713.1|
|Calculated MW||20532 Da|
|Antigen Region||109-137 aa|
|Other Names||Bcl-2-binding component 3, JFY-1, p53 up-regulated modulator of apoptosis, BBC3, PUMA|
|Target/Specificity||This BBC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 109-137 amino acids from the Central region of human BBC3.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||BBC3 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Essential mediator of p53/TP53-dependent and p53/TP53- independent apoptosis. Functions by promoting partial unfolding of BCL2L1 and dissociation of BCL2L1 from p53/TP53. Regulates ER stress-induced neuronal apoptosis.|
|Cellular Location||Mitochondrion Note=Localized to the mitochondria in order to induce cytochrome c release|
|Tissue Location||Ubiquitously expressed.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
BBC3 is an essential mediator of p53-dependent and p53-independent apoptosis.
Cazanave, S.C., et al. Am. J. Physiol. Gastrointest. Liver Physiol. 299 (1), G236-G243 (2010) :
Gurzov, E.N., et al. J. Biol. Chem. 285(26):19910-19920(2010)
Hemmati, P.G., et al. J. Mol. Med. 88(6):609-622(2010)
Gomes, N.P., et al. Genes Dev. 24(10):1022-1034(2010)
Liu, F.T., et al. Biochem. Biophys. Res. Commun. 310(3):956-962(2003)
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