|Application ||WB, IHC-P, E|
|Other Accession||Q28685, Q29243, NP_001171107.1, NP_001171111.1, NP_004384.4|
|Calculated MW||97441 Da|
|Antigen Region||718-747 aa|
|Other Names||Dystroglycan, Dystrophin-associated glycoprotein 1, Alpha-dystroglycan, Alpha-DG, Beta-dystroglycan, Beta-DG, DAG1|
|Target/Specificity||This DAG1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 718-747 amino acids from the C-terminal region of human DAG1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||DAG1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||The dystroglycan complex is involved in a number of processes including laminin and basement membrane assembly, sarcolemmal stability, cell survival, peripheral nerve myelination, nodal structure, cell migration, and epithelial polarization. Beta-dystroglycan is a transmembrane protein that plays important roles in connecting the extracellular matrix to the cytoskeleton. Acts as a cell adhesion receptor in both muscle and non-muscle tissues. Receptor for both DMD and UTRN and, through these interactions, scaffolds axin to the cytoskeleton. Also functions in cell adhesion-mediated signaling and implicated in cell polarity.|
|Cellular Location||Alpha-dystroglycan: Secreted, extracellular space|
|Tissue Location||Expressed in a variety of fetal and adult tissues. In epidermal tissue, located to the basement membrane Also expressed in keratinocytes and fibroblasts|
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Provided below are standard protocols that you may find useful for product applications.
Dystroglycan is a laminin binding component of the dystrophin-glycoprotein complex which provides a linkage between the subsarcolemmal cytoskeleton and the extracellular matrix. Dystroglycan 1 is a candidate gene for the site of the mutation in autosomal recessive muscular dystrophies. The dramatic reduction of dystroglycan 1 in Duchenne muscular dystrophy leads to a loss of linkage between the sarcolemma and extracellular matrix, rendering muscle fibers more susceptible to necrosis. Dystroglycan also functions as dual receptor for agrin and laminin-2 in the Schwann cell membrane. The muscle and nonmuscle isoforms of dystroglycan differ by carbohydrate moieties but not protein sequence. Alternative splicing results in multiple transcript variants all encoding the same protein.
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Nilsson, J., et al. Glycobiology 20(9):1160-1169(2010)
Lara-Chacon, B., et al. J. Cell. Biochem. 110(3):706-717(2010)
Sgambato, A., et al. Pathology 42(3):248-254(2010)
Masaki, T., et al. J. Biomed. Biotechnol. 2010, 740403 (2010) :
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