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GTF2E2 Antibody (C-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - GTF2E2 Antibody (C-term) AP14111b
    Western blot analysis of GTF2E2 (arrow) using rabbit polyclonal GTF2E2 Antibody (C-term) (Cat. #AP14111b). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GTF2E2 gene.
    detail
  • WB - GTF2E2 Antibody (C-term) AP14111b
    GTF2E2 Antibody (C-term) (Cat. #AP14111b) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the GTF2E2 antibody detected the GTF2E2 protein (arrow).
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P29084
Other Accession Q2KJF9, NP_002086.1
Reactivity Human
Predicted Bovine
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 33044 Da
Antigen Region 240-269 aa
Additional Information
Gene ID 2961
Other Names Transcription initiation factor IIE subunit beta, TFIIE-beta, General transcription factor IIE subunit 2, GTF2E2, TF2E2
Target/Specificity This GTF2E2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 240-269 amino acids from the C-terminal region of human GTF2E2.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGTF2E2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GTF2E2
Synonyms TF2E2
Function Recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase.
Cellular Location Nucleus.
Citations (0)
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Background

GTF2E2 recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase.

References

Cirulli, E.T., et al. Eur. J. Hum. Genet. 18(7):815-820(2010)
Festen, E.A., et al. Am. J. Gastroenterol. 105(2):395-402(2010)
Tanaka, A., et al. Genes Cells 14(3):395-405(2009)
Jawhari, A., et al. EMBO Rep. 7(5):500-505(2006)
Watanabe, T., et al. Mol. Cell. Biol. 23(8):2914-2926(2003)

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$ 182.50
$ 70.00
Cat# AP14111b
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